Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 2449470..2450097 Replicon chromosome
Accession NZ_CP046996
Organism Dehalobacter restrictus strain 12DCA

Toxin (Protein)


Gene name pemK Uniprot ID K4L4V2
Locus tag GQ588_RS12020 Protein ID WP_015043684.1
Coordinates 2449470..2449820 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1C2Y501
Locus tag GQ588_RS12025 Protein ID WP_019225279.1
Coordinates 2449810..2450097 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQ588_RS12000 2444513..2445223 - 711 WP_019225283.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
GQ588_RS12005 2445296..2445721 - 426 WP_019225282.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
GQ588_RS12010 2445739..2446962 - 1224 WP_019225281.1 amidohydrolase family protein -
GQ588_RS12015 2447160..2449298 - 2139 WP_019225280.1 RNA-binding transcriptional accessory protein -
GQ588_RS12020 2449470..2449820 - 351 WP_015043684.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GQ588_RS12025 2449810..2450097 - 288 WP_019225279.1 hypothetical protein Antitoxin
GQ588_RS12030 2450257..2451057 - 801 WP_019225278.1 glutamine amidotransferase -
GQ588_RS12035 2451054..2452481 - 1428 WP_019225277.1 Mur ligase family protein -
GQ588_RS12040 2452726..2453274 + 549 WP_019225276.1 gamma carbonic anhydrase family protein -
GQ588_RS12045 2453341..2453781 - 441 WP_019225275.1 MOSC domain-containing protein -
GQ588_RS12050 2453823..2454332 - 510 WP_019225274.1 molybdopterin-guanine dinucleotide biosynthesis protein B -
GQ588_RS12055 2454407..2454823 - 417 WP_019225273.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-38)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12801.78 Da        Isoelectric Point: 5.2375

>T145253 WP_015043684.1 NZ_CP046996:c2449820-2449470 [Dehalobacter restrictus]
MSIKRGEIFYAELNPVVGSEQGGTRPVLVIQNDIGNQYSPTTIIAAITSQISKAKLPTHVEVKAKRSGLERDSVILTEQI
RTIDKSRLKEKVAVLDEEVMLRVDEAIEISLGLTEI

Download         Length: 351 bp

>T145253 NZ_CP062344:1203065-1203253 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10996.59 Da        Isoelectric Point: 4.3854

>AT145253 WP_019225279.1 NZ_CP046996:c2450097-2449810 [Dehalobacter restrictus]
MAETRRIVVCLPESIIEEVDEIVSSEKLNRSDFIKEAVYSVLIERRKAGIREQMRQGYVEMAQINLSMAVDLCQAEEEAT
MRYEGKLAWSVGYEY

Download         Length: 288 bp

>AT145253 NZ_CP062344:1202776-1203036 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K4L4V2

Antitoxin

Source ID Structure
AlphaFold DB A0A1C2Y501

References