Detailed information of TA system TA145251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 4940547..4941106
• Replicon: chromosome
• Accession: NZ_CP046973
• Organism: Microcystis aeruginosa FD4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GQR42_RS24465
• Protein ID: WP_158201966.1
• Coordinates: 4940547..4940885 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GQR42_RS24470
• Protein ID: WP_158201967.1
• Coordinates: 4940882..4941106 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQR42_RS24430	4936180..4936776	+	597	WP_158201961.1	NADH-quinone oxidoreductase subunit J	-
GQR42_RS24435	4936802..4937107	+	306	WP_197533180.1	NADH-quinone oxidoreductase subunit NuoK	-
GQR42_RS24440	4937348..4937494	-	147	WP_158201962.1	hypothetical protein	-
GQR42_RS24445	4937692..4938320	-	629	Protein_4857	DUF1361 domain-containing protein	-
GQR42_RS24450	4938324..4938740	-	417	WP_158201963.1	acyl-CoA thioesterase	-
GQR42_RS24455	4938748..4939266	-	519	WP_158201964.1	cofactor assembly of complex C subunit B	-
GQR42_RS24460	4939402..4940307	+	906	WP_158201965.1	metal ABC transporter permease	-
GQR42_RS24465	4940547..4940885	-	339	WP_158201966.1	DUF5615 family PIN-like protein	Toxin
GQR42_RS24470	4940882..4941106	-	225	WP_158201967.1	DUF433 domain-containing protein	Antitoxin
GQR42_RS24475	4941371..4941595	+	225	WP_158201968.1	hypothetical protein	-
GQR42_RS24480	4941686..4942291	-	606	WP_158201969.1	DedA family protein	-
GQR42_RS24485	4942446..4943723	-	1278	WP_158201970.1	adenosylhomocysteinase	-
GQR42_RS24490	4943868..4944539	-	672	WP_158201971.1	riboflavin synthase	-
GQR42_RS24495	4944555..4945850	-	1296	WP_158201972.1	glutamate-5-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-78)

Antitoxin

• Protein of unknown function DUF433 (8-63)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12320.12 Da
• Isoelectric Point: 4.3023

>T145251 WP_158201966.1 NZ_CP046973:c4940885-4940547 [Microcystis aeruginosa FD4]
MTIWVDAHLSPAIATWISTTLEIEAVALRDLGLRDAEDTEIFQVAKARRAILMTKDSDFVDLVERLGSPPQIIWLTCGNT
SNARLREILSETLSRALELLAAGETLVEISGN

Nucleotide

• Length: 339 bp

>T145251 NZ_CP062344:120647-120799 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGACTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8021.39 Da
• Isoelectric Point: 5.7582

>AT145251 WP_158201967.1 NZ_CP046973:c4941106-4940882 [Microcystis aeruginosa FD4]
MAELLSRITVNPRQCGGRACIRGMRIRVSDVLDLFAAGLSAGEILDEMPDLEADDLKAALLYASRKLNHPVLVA

Nucleotide

• Length: 225 bp

>AT145251 NZ_CP062344:120023-120622 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA