Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 138525..139168 Replicon plasmid pKPC2_020130
Accession NZ_CP046963
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020130

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CLQ86_RS27880 Protein ID WP_001044770.1
Coordinates 138752..139168 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CLQ86_RS27875 Protein ID WP_001261282.1
Coordinates 138525..138755 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ86_RS27840 (133748) 133748..134527 - 780 WP_013214009.1 site-specific integrase -
CLQ86_RS27845 (134711) 134711..135715 - 1005 WP_011977814.1 hypothetical protein -
CLQ86_RS27850 (135745) 135745..135948 - 204 WP_011977813.1 hypothetical protein -
CLQ86_RS27855 (135994) 135994..136515 - 522 WP_013214008.1 hypothetical protein -
CLQ86_RS27860 (136573) 136573..136965 - 393 WP_011977811.1 hypothetical protein -
CLQ86_RS27865 (137002) 137002..137946 - 945 WP_011977810.1 hypothetical protein -
CLQ86_RS27870 (138107) 138107..138568 - 462 WP_014343465.1 hypothetical protein -
CLQ86_RS27875 (138525) 138525..138755 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CLQ86_RS27880 (138752) 138752..139168 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CLQ86_RS27885 (139242) 139242..140804 + 1563 WP_004206609.1 AAA family ATPase -
CLQ86_RS27890 (140789) 140789..141811 + 1023 WP_000361404.1 helicase UvrD -
CLQ86_RS27895 (142067) 142067..142764 + 698 WP_223174630.1 IS1 family transposase -
CLQ86_RS27900 (142793) 142793..143065 + 273 Protein_185 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB - 1..152833 152833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T145242 WP_001044770.1 NZ_CP046963:138752-139168 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T145242 NZ_CP062342:c167903-167808 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT145242 WP_001261282.1 NZ_CP046963:138525-138755 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT145242 NZ_CP062342:167723-167780 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References