Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5391100..5391725 Replicon chromosome
Accession NZ_CP046962
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020130

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag CLQ86_RS26610 Protein ID WP_002882817.1
Coordinates 5391100..5391483 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag CLQ86_RS26615 Protein ID WP_004150355.1
Coordinates 5391483..5391725 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ86_RS26595 (CLQ86_26135) 5388466..5389368 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
CLQ86_RS26600 (CLQ86_26140) 5389365..5390000 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
CLQ86_RS26605 (CLQ86_26145) 5389997..5390926 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
CLQ86_RS26610 (CLQ86_26150) 5391100..5391483 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
CLQ86_RS26615 (CLQ86_26155) 5391483..5391725 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
CLQ86_RS26620 (CLQ86_26160) 5391930..5392847 + 918 WP_002882812.1 alpha/beta hydrolase -
CLQ86_RS26625 (CLQ86_26165) 5392861..5393802 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
CLQ86_RS26630 (CLQ86_26170) 5393847..5394284 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
CLQ86_RS26635 (CLQ86_26175) 5394281..5395141 - 861 WP_002882807.1 virulence factor BrkB family protein -
CLQ86_RS26640 (CLQ86_26180) 5395135..5395734 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T145238 WP_002882817.1 NZ_CP046962:c5391483-5391100 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T145238 NZ_CP062340:2674999-2675208 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT145238 WP_004150355.1 NZ_CP046962:c5391725-5391483 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT145238 NZ_CP062340:2674694-2674987 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References