Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4807538..4808348 Replicon chromosome
Accession NZ_CP046962
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020130

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag CLQ86_RS23810 Protein ID WP_002887280.1
Coordinates 4807538..4808071 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag CLQ86_RS23815 Protein ID WP_002887278.1
Coordinates 4808082..4808348 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ86_RS23805 (CLQ86_23380) 4806369..4807490 + 1122 WP_002887282.1 cupin domain-containing protein -
CLQ86_RS23810 (CLQ86_23385) 4807538..4808071 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
CLQ86_RS23815 (CLQ86_23390) 4808082..4808348 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
CLQ86_RS23820 (CLQ86_23395) 4808451..4809884 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
CLQ86_RS23825 (CLQ86_23400) 4809874..4810557 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
CLQ86_RS23830 (CLQ86_23405) 4810729..4812114 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
CLQ86_RS23835 (CLQ86_23410) 4812132..4812476 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T145235 WP_002887280.1 NZ_CP046962:c4808071-4807538 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T145235 NZ_CP062340:2339415-2339522 [Staphylococcus aureus]
ATCTTCAATTTATTAATTGACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCAAATTGGTTACGAAC
GCGCAGCAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT145235 WP_002887278.1 NZ_CP046962:c4808348-4808082 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT145235 NZ_CP062340:c2339599-2339539 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References