Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 743321..744096 Replicon chromosome
Accession NZ_CP046962
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020130

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag CLQ86_RS03715 Protein ID WP_004150910.1
Coordinates 743611..744096 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag CLQ86_RS03710 Protein ID WP_004150912.1
Coordinates 743321..743614 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ86_RS03690 (CLQ86_03650) 738452..739363 + 912 WP_004188550.1 LysR family transcriptional regulator -
CLQ86_RS03695 (CLQ86_03655) 739364..740512 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
CLQ86_RS03700 (CLQ86_03660) 740523..741899 - 1377 WP_004217775.1 cystathionine beta-synthase -
CLQ86_RS03710 (CLQ86_03670) 743321..743614 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
CLQ86_RS03715 (CLQ86_03675) 743611..744096 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
CLQ86_RS03720 (CLQ86_03680) 744800..745393 + 594 WP_004188553.1 hypothetical protein -
CLQ86_RS03725 (CLQ86_03685) 745490..745706 + 217 Protein_732 transposase -
CLQ86_RS03730 (CLQ86_03700) 746312..747184 + 873 WP_004188557.1 ParA family protein -
CLQ86_RS03735 (CLQ86_03705) 747184..747567 + 384 WP_004150906.1 hypothetical protein -
CLQ86_RS03740 (CLQ86_03710) 747560..748927 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 742278..745642 3364


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T145223 WP_004150910.1 NZ_CP046962:743611-744096 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T145223 NZ_CP062338:2273490-2273756 [Staphylococcus aureus]
ATGAGCAATTATACGGTTAAGATTAAAAATTCGGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAAAAGCC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACTGTAGATGATCGAAATAAAGAAGTATTAATA
TTATCGGCGTGGTCACATTATGATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT145223 WP_004150912.1 NZ_CP046962:743321-743614 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT145223 NZ_CP062338:2273233-2273490 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGATGATTGGAAAAGTATACAAGAAACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGATAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References