Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 329130..329716 Replicon chromosome
Accession NZ_CP046962
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020130

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag CLQ86_RS01530 Protein ID WP_002920800.1
Coordinates 329348..329716 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag CLQ86_RS01525 Protein ID WP_002920802.1
Coordinates 329130..329351 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ86_RS01505 (CLQ86_01485) 325287..326213 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
CLQ86_RS01510 (CLQ86_01490) 326210..327487 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
CLQ86_RS01515 (CLQ86_01495) 327484..328251 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
CLQ86_RS01520 (CLQ86_01500) 328253..328966 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
CLQ86_RS01525 (CLQ86_01505) 329130..329351 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CLQ86_RS01530 (CLQ86_01510) 329348..329716 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
CLQ86_RS01535 (CLQ86_01515) 329989..331305 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
CLQ86_RS01540 (CLQ86_01520) 331412..332299 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
CLQ86_RS01545 (CLQ86_01525) 332296..333141 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
CLQ86_RS01550 (CLQ86_01530) 333143..334213 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 324116..334950 10834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T145221 WP_002920800.1 NZ_CP046962:329348-329716 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T145221 NZ_CP062338:c165406-165311 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT145221 WP_002920802.1 NZ_CP046962:329130-329351 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT145221 NZ_CP062338:165226-165283 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References