Detailed information of TA system TA145220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 3894..4474
• Replicon: plasmid p2_015247
• Accession: NZ_CP046960
• Organism: Klebsiella pneumoniae strain WCHKP015247

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: C6M24_RS27985
• Protein ID: WP_071177730.1
• Coordinates: 4160..4474 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: C6M24_RS27980
• Protein ID: WP_000093040.1
• Coordinates: 3894..4172 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6M24_RS27960 (C6M24_27480)	335..1822	-	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
C6M24_RS27965 (C6M24_27485)	2467..2712	+	246	WP_032440458.1	hypothetical protein	-
C6M24_RS27970 (C6M24_27490)	2986..3357	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
C6M24_RS27975	3354..3719	+	366	WP_072354022.1	TonB family protein	-
C6M24_RS27980 (C6M24_27500)	3894..4172	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
C6M24_RS27985 (C6M24_27505)	4160..4474	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6M24_RS27990 (C6M24_27510)	4638..5066	+	429	WP_001140599.1	hypothetical protein	-
C6M24_RS27995 (C6M24_27515)	5092..5271	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
C6M24_RS28000 (C6M24_27520)	5298..5828	-	531	WP_071177729.1	hypothetical protein	-
C6M24_RS28005 (C6M24_27525)	5835..6566	-	732	WP_071177728.1	MobC family replication-relaxation protein	-
C6M24_RS28010 (C6M24_27530)	6566..8530	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..12746	12746

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T145220 WP_071177730.1 NZ_CP046960:4160-4474 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T145220 NZ_CP062338:119085-119237 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT145220 WP_000093040.1 NZ_CP046960:3894-4172 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT145220 NZ_CP062338:118461-119060 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1