Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 47408..48051 Replicon plasmid p1_015247
Accession NZ_CP046959
Organism Klebsiella pneumoniae strain WCHKP015247

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag C6M24_RS27735 Protein ID WP_001044770.1
Coordinates 47408..47824 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag C6M24_RS27740 Protein ID WP_001261282.1
Coordinates 47821..48051 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M24_RS27715 (C6M24_27250) 43511..43783 - 273 Protein_58 transposase -
C6M24_RS27725 (C6M24_27260) 44765..45787 - 1023 WP_000361404.1 helicase UvrD -
C6M24_RS27730 (C6M24_27265) 45772..47334 - 1563 WP_004206609.1 AAA family ATPase -
C6M24_RS27735 (C6M24_27270) 47408..47824 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
C6M24_RS27740 (C6M24_27275) 47821..48051 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
C6M24_RS27745 48008..48469 + 462 WP_014343465.1 hypothetical protein -
C6M24_RS27750 (C6M24_27280) 48630..49574 + 945 WP_011977810.1 hypothetical protein -
C6M24_RS27755 (C6M24_27285) 49611..50003 + 393 WP_011977811.1 hypothetical protein -
C6M24_RS27760 (C6M24_27290) 50061..50582 + 522 WP_013214008.1 hypothetical protein -
C6M24_RS27765 (C6M24_27295) 50628..50831 + 204 WP_011977813.1 hypothetical protein -
C6M24_RS27770 (C6M24_27300) 50861..51865 + 1005 WP_011977814.1 hypothetical protein -
C6M24_RS27775 (C6M24_27305) 52049..52828 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr - 1..80768 80768


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T145219 WP_001044770.1 NZ_CP046959:c47824-47408 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T145219 NZ_CP062338:18899-19075 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGTTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT145219 WP_001261282.1 NZ_CP046959:c48051-47821 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT145219 NZ_CP062338:c19237-19063 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References