Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5369310..5369935 Replicon chromosome
Accession NZ_CP046957
Organism Klebsiella pneumoniae strain WCHKP015247

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag C6M24_RS26470 Protein ID WP_002882817.1
Coordinates 5369310..5369693 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag C6M24_RS26475 Protein ID WP_004150355.1
Coordinates 5369693..5369935 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M24_RS26455 (C6M24_26005) 5366676..5367578 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
C6M24_RS26460 (C6M24_26010) 5367575..5368210 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
C6M24_RS26465 (C6M24_26015) 5368207..5369136 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
C6M24_RS26470 (C6M24_26020) 5369310..5369693 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
C6M24_RS26475 (C6M24_26025) 5369693..5369935 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
C6M24_RS26480 (C6M24_26030) 5370140..5371057 + 918 WP_002882812.1 alpha/beta hydrolase -
C6M24_RS26485 (C6M24_26035) 5371071..5372012 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
C6M24_RS26490 (C6M24_26040) 5372057..5372494 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
C6M24_RS26495 (C6M24_26045) 5372491..5373351 - 861 WP_002882807.1 virulence factor BrkB family protein -
C6M24_RS26500 (C6M24_26050) 5373345..5373944 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T145214 WP_002882817.1 NZ_CP046957:c5369693-5369310 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T145214 NZ_CP062336:c1804933-1804829 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT145214 WP_004150355.1 NZ_CP046957:c5369935-5369693 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT145214 NZ_CP062336:1804736-1804774 [Staphylococcus aureus]
AACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References