Detailed information of TA system TA145196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 295358..295994
• Replicon: chromosome
• Accession: NZ_CP046956
• Organism: Sediminibacillus dalangtanensis strain DP4-553-S

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A1G9WRC4
• Locus tag: ERJ70_RS01580
• Protein ID: WP_074600801.1
• Coordinates: 295644..295994 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: ERJ70_RS01575
• Protein ID: WP_026571355.1
• Coordinates: 295358..295639 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERJ70_RS01555 (ERJ70_01550)	290557..290913	+	357	WP_209366699.1	holo-ACP synthase	-
ERJ70_RS01560 (ERJ70_01555)	291063..292577	+	1515	WP_209366701.1	NAD(P)H-hydrate dehydratase	-
ERJ70_RS01565 (ERJ70_01560)	292634..293653	+	1020	WP_209366703.1	outer membrane lipoprotein carrier protein LolA	-
ERJ70_RS01570 (ERJ70_01565)	294018..295172	+	1155	WP_245208091.1	alanine racemase	-
ERJ70_RS01575 (ERJ70_01570)	295358..295639	+	282	WP_026571355.1	hypothetical protein	Antitoxin
ERJ70_RS01580 (ERJ70_01575)	295644..295994	+	351	WP_074600801.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ERJ70_RS01585 (ERJ70_01580)	296306..297178	+	873	WP_209366706.1	RsbT co-antagonist protein RsbRA	-
ERJ70_RS01590 (ERJ70_01585)	297187..297543	+	357	WP_026571352.1	STAS domain-containing protein	-
ERJ70_RS01595 (ERJ70_01590)	297546..297947	+	402	WP_074600594.1	anti-sigma regulatory factor	-
ERJ70_RS01600 (ERJ70_01595)	297961..298971	+	1011	WP_209366708.1	PP2C family protein-serine/threonine phosphatase	-
ERJ70_RS01605 (ERJ70_01600)	299034..299363	+	330	WP_209366710.1	STAS domain-containing protein	-
ERJ70_RS01610 (ERJ70_01605)	299365..299841	+	477	WP_209366712.1	anti-sigma B factor RsbW	-
ERJ70_RS01615 (ERJ70_01610)	299813..300601	+	789	WP_074600590.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13000.12 Da
• Isoelectric Point: 7.3182

>T145196 WP_074600801.1 NZ_CP046956:295644-295994 [Sediminibacillus dalangtanensis]
MIVKRGEVYFADLSPVVGSEQGGVRPVLVLQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDKHMMENINKALEISLGLTDF

Nucleotide

• Length: 351 bp

>T145196 NZ_CP062331:2725588-2725797 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10664.17 Da
• Isoelectric Point: 4.5915

>AT145196 WP_026571355.1 NZ_CP046956:295358-295639 [Sediminibacillus dalangtanensis]
MVVSESLQEIIVRLPKNLLSEVDGLMETENSDLSDFICQATKSYLREKKKRHIRESMQRGYMEMAKINLNIASEAFQAEE
EAENTLERLVSGV

Nucleotide

• Length: 282 bp

>AT145196 NZ_CP062331:2725283-2725576 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A1G9WRC4