Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5326552..5327177 Replicon chromosome
Accession NZ_CP046949
Organism Klebsiella pneumoniae strain BD_DM_914

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GQY16_RS25695 Protein ID WP_002882817.1
Coordinates 5326552..5326935 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GQY16_RS25700 Protein ID WP_004150355.1
Coordinates 5326935..5327177 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQY16_RS25680 5323918..5324820 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GQY16_RS25685 5324817..5325452 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GQY16_RS25690 5325449..5326378 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GQY16_RS25695 5326552..5326935 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GQY16_RS25700 5326935..5327177 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GQY16_RS25705 5327382..5328299 + 918 WP_004181612.1 alpha/beta hydrolase -
GQY16_RS25710 5328313..5329254 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GQY16_RS25715 5329299..5329736 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GQY16_RS25720 5329733..5330593 - 861 WP_004146232.1 virulence factor BrkB family protein -
GQY16_RS25725 5330587..5331186 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T145195 WP_002882817.1 NZ_CP046949:c5326935-5326552 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T145195 NZ_CP062331:2668224-2668328 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTTATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT145195 WP_004150355.1 NZ_CP046949:c5327177-5326935 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT145195 NZ_CP062331:c2668487-2668323 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCATTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References