Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5281158..5281783 Replicon chromosome
Accession NZ_CP046939
Organism Klebsiella pneumoniae strain BD_DM_697

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GQY17_RS25365 Protein ID WP_002882817.1
Coordinates 5281158..5281541 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GQY17_RS25370 Protein ID WP_004150355.1
Coordinates 5281541..5281783 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQY17_RS25350 5278524..5279426 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GQY17_RS25355 5279423..5280058 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GQY17_RS25360 5280055..5280984 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GQY17_RS25365 5281158..5281541 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GQY17_RS25370 5281541..5281783 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GQY17_RS25375 5281988..5282905 + 918 WP_004181612.1 alpha/beta hydrolase -
GQY17_RS25380 5282919..5283860 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GQY17_RS25385 5283905..5284342 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GQY17_RS25390 5284339..5285199 - 861 WP_004146232.1 virulence factor BrkB family protein -
GQY17_RS25395 5285193..5285792 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T145175 WP_002882817.1 NZ_CP046939:c5281541-5281158 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T145175 NZ_CP062326:2632192-2632401 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAAAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT145175 WP_004150355.1 NZ_CP046939:c5281783-5281541 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT145175 NZ_CP062326:2631887-2632180 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAAAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CATTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References