Detailed information of TA system TA145174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4968050..4969731
• Replicon: chromosome
• Accession: NZ_CP046939
• Organism: Klebsiella pneumoniae strain BD_DM_697

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: GQY17_RS23935
• Protein ID: WP_004178299.1
• Coordinates: 4968050..4968967 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: GQY17_RS23940
• Protein ID: WP_004177809.1
• Coordinates: 4968973..4969731 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQY17_RS23915	4963356..4964876	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
GQY17_RS23920	4964873..4965871	+	999	WP_002885172.1	ribose ABC transporter permease	-
GQY17_RS23925	4965902..4966843	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
GQY17_RS23930	4966918..4967925	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
GQY17_RS23935	4968050..4968967	-	918	WP_004178299.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GQY17_RS23940	4968973..4969731	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GQY17_RS23945	4969817..4970545	-	729	WP_004178298.1	response regulator transcription factor	-
GQY17_RS23950	4970818..4972965	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
GQY17_RS28785	4972962..4973105	-	144	WP_022631365.1	hypothetical protein	-
GQY17_RS23955	4973543..4974229	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34649.06 Da
• Isoelectric Point: 5.4707

>T145174 WP_004178299.1 NZ_CP046939:c4968967-4968050 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPSQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T145174 NZ_CP062326:2289192-2289299 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT145174 WP_004177809.1 NZ_CP046939:c4969731-4968973 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT145174 NZ_CP062326:c2289376-2289316 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Structures

Antitoxin

• AlphaFold DB: A6TH05