Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3358130..3358767 Replicon chromosome
Accession NZ_CP046918
Organism Bacillus velezensis strain BA-26

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GPY14_RS16070 Protein ID WP_003156187.1
Coordinates 3358417..3358767 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag GPY14_RS16065 Protein ID WP_003156188.1
Coordinates 3358130..3358411 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPY14_RS16045 3354490..3355089 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
GPY14_RS16050 3355182..3355547 + 366 WP_007410229.1 holo-ACP synthase -
GPY14_RS16055 3355712..3356724 + 1013 Protein_3135 outer membrane lipoprotein carrier protein LolA -
GPY14_RS16060 3356841..3358010 + 1170 WP_039252648.1 alanine racemase -
GPY14_RS16065 3358130..3358411 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GPY14_RS16070 3358417..3358767 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GPY14_RS16075 3358885..3359706 + 822 WP_003156182.1 STAS domain-containing protein -
GPY14_RS16080 3359711..3360076 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
GPY14_RS16085 3360079..3360480 + 402 WP_003156178.1 anti-sigma regulatory factor -
GPY14_RS16090 3360492..3361499 + 1008 WP_039252645.1 PP2C family protein-serine/threonine phosphatase -
GPY14_RS16095 3361563..3361892 + 330 WP_031378636.1 anti-sigma factor antagonist -
GPY14_RS16100 3361889..3362371 + 483 WP_007609591.1 anti-sigma B factor RsbW -
GPY14_RS16105 3362337..3363125 + 789 WP_158186005.1 RNA polymerase sigma factor SigB -
GPY14_RS16110 3363125..3363727 + 603 WP_039252641.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T145156 WP_003156187.1 NZ_CP046918:3358417-3358767 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T145156 NZ_CP062322:2373593-2373859 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT145156 WP_003156188.1 NZ_CP046918:3358130-3358411 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT145156 NZ_CP062322:2373342-2373593 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References