145134

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 99210..99903 Replicon plasmid p1_PM101005
Accession NZ_CP046903
Organism Pseudomonas stutzeri strain PM101005

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag GQA94_RS22420 Protein ID WP_003151133.1
Coordinates 99526..99903 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag GQA94_RS22415 Protein ID WP_001172026.1
Coordinates 99210..99545 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQA94_RS22375 95048..95962 - 915 WP_047883045.1 acetaldehyde dehydrogenase (acetylating) -
GQA94_RS22380 95959..96168 - 210 WP_047883046.1 2-hydroxymuconate tautomerase family protein -
GQA94_RS22385 96183..96578 - 396 Protein_111 4-oxalocrotonate decarboxylase -
GQA94_RS22390 96746..97660 - 915 WP_001736514.1 HD domain-containing protein -
GQA94_RS22395 97707..98015 - 309 WP_158190220.1 DUF3391 domain-containing protein -
GQA94_RS22400 98133..98513 - 381 WP_001054412.1 hypothetical protein -
GQA94_RS22405 98510..98836 - 327 WP_047883117.1 hypothetical protein -
GQA94_RS22410 98860..99195 - 336 WP_000741275.1 hypothetical protein -
GQA94_RS22415 99210..99545 - 336 WP_001172026.1 helix-turn-helix domain-containing protein Antitoxin
GQA94_RS22420 99526..99903 - 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GQA94_RS22425 100152..101831 + 1680 WP_003465068.1 DDE-type integrase/transposase/recombinase -
GQA94_RS22430 101834..102742 + 909 WP_003465065.1 TniB family NTP-binding protein -
GQA94_RS22435 102739..103956 + 1218 WP_054471483.1 TniQ family protein -
GQA94_RS22440 104017..104631 + 615 WP_000904941.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..265581 265581
- flank IS/Tn - - 104017..104631 614


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T145134 WP_003151133.1 NZ_CP046903:c99903-99526 [Pseudomonas stutzeri]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T145134 NZ_CP062318:2632401-2632610 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT145134 WP_001172026.1 NZ_CP046903:c99545-99210 [Pseudomonas stutzeri]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT145134 NZ_CP062318:2632096-2632389 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References