Detailed information of TA system TA145125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5737828..5738350
• Replicon: chromosome
• Accession: NZ_CP046874
• Organism: Pseudomonas mediterranea strain S58

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: E3Z27_RS25015
• Protein ID: WP_159286032.1
• Coordinates: 5738057..5738350 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A6I6QD85
• Locus tag: E3Z27_RS25010
• Protein ID: WP_047702740.1
• Coordinates: 5737828..5738067 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z27_RS24985	5733109..5734446	+	1338	WP_159286031.1	phosphatase PAP2/dual specificity phosphatase family protein	-
E3Z27_RS24990	5734436..5734885	+	450	WP_047702736.1	hypothetical protein	-
E3Z27_RS24995	5735107..5735727	+	621	WP_047702737.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
E3Z27_RS25000	5735727..5736659	+	933	WP_047702738.1	phosphatidate cytidylyltransferase	-
E3Z27_RS25005	5736720..5737610	-	891	WP_047702739.1	MBL fold metallo-hydrolase	-
E3Z27_RS25010	5737828..5738067	+	240	WP_047702740.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
E3Z27_RS25015	5738057..5738350	+	294	WP_159286032.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3Z27_RS25020	5738407..5739249	-	843	WP_041021241.1	DUF2135 domain-containing protein	-
E3Z27_RS25025	5739253..5740872	-	1620	WP_047702742.1	DUF2300 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-81)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11618.43 Da
• Isoelectric Point: 9.7094

>T145125 WP_159286032.1 NZ_CP046874:5738057-5738350 [Pseudomonas mediterranea]
MTYSLEFDRRALKEWNKLGDTLRQQFKKKLIEVLENPRIEANRLRQLPDCYKLKLRSAGYRLIYQVIDQEVVVFVVAIGK
REREAVYQDVQERISSD

Nucleotide

• Length: 294 bp

>T145125 NZ_CP062314:2785097-2785459 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8896.21 Da
• Isoelectric Point: 4.6017

>AT145125 WP_047702740.1 NZ_CP046874:5737828-5738067 [Pseudomonas mediterranea]
MASINIRIDDDLKQRSFAELEKLGVTPSELLRQTLQYVAERGKLPFKAALLSEEDEALIAVVTERLSNPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT145125 NZ_CP062314:2784930-2785100 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6I6QD85