Detailed information of TA system TA145122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 490974..491563
• Replicon: chromosome
• Accession: NZ_CP046874
• Organism: Pseudomonas mediterranea strain S58

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: E3Z27_RS02150
• Protein ID: WP_081993227.1
• Coordinates: 490974..491279 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E3Z27_RS02155
• Protein ID: WP_047700469.1
• Coordinates: 491276..491563 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z27_RS02125	486063..486908	+	846	WP_159284831.1	general secretion pathway protein GspK	-
E3Z27_RS02130	486908..488011	+	1104	WP_055127145.1	type II secretory protein pull	-
E3Z27_RS02135	488011..488466	+	456	WP_055127146.1	type II secretion system protein M	-
E3Z27_RS02140	488619..489896	-	1278	WP_047700472.1	MFS transporter	-
E3Z27_RS02145	490016..490951	+	936	WP_047700471.1	LysR family transcriptional regulator	-
E3Z27_RS02150	490974..491279	+	306	WP_081993227.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3Z27_RS02155	491276..491563	+	288	WP_047700469.1	putative addiction module antidote protein	Antitoxin
E3Z27_RS02160	491611..495628	-	4018	Protein_422	hypothetical protein	-
E3Z27_RS02165	495939..496385	-	447	WP_047700467.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-91)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11432.14 Da
• Isoelectric Point: 10.0053

>T145122 WP_081993227.1 NZ_CP046874:490974-491279 [Pseudomonas mediterranea]
MTEIIRSCTFSGWLSKLADSRARIRIQVRIDRMADGNLGDVKAIGEGLSEARIDYGPGYRIYFMQQGRQLIILLCGGDKS
SQTRDIKQARLIAKSWQEQNP

Nucleotide

• Length: 306 bp

>T145122 NZ_CP062314:2441916-2442182 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10413.73 Da
• Isoelectric Point: 4.6412

>AT145122 WP_047700469.1 NZ_CP046874:491276-491563 [Pseudomonas mediterranea]
MTEQFTRWDSAEYLKTEEDMANYLDACMEEAGDDPAFIAKALGTIARARGMSQVARDAGLSRENLYRALSGEGNPEFGTI
LKVVRALGLKLHAST

Nucleotide

• Length: 288 bp

>AT145122 NZ_CP062314:2441665-2441916 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGTTATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA