Detailed information of TA system TA145120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 74703..75301
• Replicon: plasmid unnamed
• Accession: NZ_CP046873
• Organism: Pseudomonas putida strain MX-2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A6H1Q9F7
• Locus tag: GPM17_RS27565
• Protein ID: WP_028692294.1
• Coordinates: 74999..75301 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GPM17_RS27560
• Protein ID: WP_028692295.1
• Coordinates: 74703..75002 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPM17_RS27540	70075..71357	-	1283	Protein_88	Y-family DNA polymerase	-
GPM17_RS27545	71347..71775	-	429	WP_028691923.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
GPM17_RS27550	73844..74167	-	324	WP_028692297.1	hypothetical protein	-
GPM17_RS27555	74462..74683	-	222	WP_028692296.1	hypothetical protein	-
GPM17_RS27560	74703..75002	-	300	WP_028692295.1	putative addiction module antidote protein	Antitoxin
GPM17_RS27565	74999..75301	-	303	WP_028692294.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPM17_RS27570	75504..76283	+	780	WP_028692293.1	DUF4942 domain-containing protein	-
GPM17_RS27575	76436..76804	+	369	WP_028692292.1	hypothetical protein	-
GPM17_RS27580	76831..77148	+	318	WP_028692291.1	hypothetical protein	-
GPM17_RS27585	77486..78577	+	1092	WP_051555741.1	hypothetical protein	-
GPM17_RS27590	78723..78965	+	243	WP_028692289.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aac(6')-Ib-cr / qnrVC1 / blaOXA-10 / ant(3'')-Ia / dfrA14 / aph(3'')-Ib / aph(6)-Id / aac(3)-IId	-	1..103556	103556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (7-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11277.85 Da
• Isoelectric Point: 10.3903

>T145120 WP_028692294.1 NZ_CP046873:c75301-74999 [Pseudomonas putida]
MKTIKQTATYRNWERKLRDKQAKAIIAARVFRVAHGLLGDVQPVGQGISELRIHHGPGYRVYFQQRGNQLVLLLCGGDKS
SQARDIETAKSLASQWSDDE

Nucleotide

• Length: 303 bp

>T145120 NZ_CP062314:2385128-2385394 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10825.27 Da
• Isoelectric Point: 5.1084

>AT145120 WP_028692295.1 NZ_CP046873:c75002-74703 [Pseudomonas putida]
MKEQFYDYDPADALTDAESIAVFMEDAFETGDAAYIAKCLGVVARAKGMTALANESGLSREHLYRSFSDKGNPTLKTLLA
VMRAVGLDLTTRKHVEPAH

Nucleotide

• Length: 300 bp

>AT145120 NZ_CP062314:2384871-2385128 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: A0A6H1Q9F7