Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 2213051..2213934 Replicon chromosome
Accession NZ_CP046872
Organism Pseudomonas putida strain MX-2

Toxin (Protein)


Gene name - Uniprot ID A0A173HDN1
Locus tag GPM17_RS09990 Protein ID WP_019751987.1
Coordinates 2213497..2213934 (+) Length 146 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID I7C3Q7
Locus tag GPM17_RS09985 Protein ID WP_003250525.1
Coordinates 2213051..2213500 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPM17_RS09960 2208474..2209673 + 1200 WP_003250514.1 sugar transporter -
GPM17_RS09965 2209713..2210297 - 585 WP_060537711.1 LysE family transporter -
GPM17_RS09970 2210349..2211179 - 831 WP_003250518.1 AraC family transcriptional regulator -
GPM17_RS09975 2211228..2212151 - 924 WP_004573727.1 LysR family transcriptional regulator -
GPM17_RS09980 2212255..2212908 + 654 WP_185280194.1 oxygen-insensitive NAD(P)H nitroreductase -
GPM17_RS09985 2213051..2213500 + 450 WP_003250525.1 DUF2384 domain-containing protein Antitoxin
GPM17_RS09990 2213497..2213934 + 438 WP_019751987.1 RES family NAD+ phosphorylase Toxin
GPM17_RS09995 2213992..2215146 - 1155 WP_185280195.1 aminotransferase class V-fold PLP-dependent enzyme -
GPM17_RS10000 2215340..2216263 - 924 WP_004573723.1 LysR family transcriptional regulator -
GPM17_RS10005 2216404..2217633 + 1230 WP_064492457.1 acyl-CoA dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-138)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(33-91)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15953.22 Da        Isoelectric Point: 6.0704

>T145117 WP_019751987.1 NZ_CP046872:2213497-2213934 [Pseudomonas putida]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPPGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSAHPELTRTLGNRFLDDGSALLLPVPSAIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF

Download         Length: 438 bp

>T145117 NZ_CP062314:122119-122271 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16956.46 Da        Isoelectric Point: 5.7258

>AT145117 WP_003250525.1 NZ_CP046872:2213051-2213500 [Pseudomonas putida]
MLAEVLRDNGYHEYRARLQALLDIPELASDFEIHTRITDGFAATWLVKLTERGVLTPVERDQIIPLRTLKSRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGYGL

Download         Length: 450 bp

>AT145117 NZ_CP062314:121495-122094 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A173HDN1

Antitoxin

Source ID Structure
AlphaFold DB I7C3Q7

References