Detailed information of TA system TA145073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 423194..425019
• Replicon: chromosome
• Accession: NZ_CP046844
• Organism: Vibrio cholerae C6706

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: GPY04_RS01970
• Protein ID: WP_000392003.1
• Coordinates: 423658..425019 (-)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GPY04_RS01965
• Protein ID: WP_000804849.1
• Coordinates: 423194..423661 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY04_RS01950	418474..419178	-	705	WP_001880605.1	NERD domain-containing protein	-
GPY04_RS01955	419248..422184	-	2937	WP_001283872.1	DEAD/DEAH box helicase family protein	-
GPY04_RS01960	422188..422766	-	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
GPY04_RS01965	423194..423661	-	468	WP_000804849.1	helix-turn-helix domain-containing protein	Antitoxin
GPY04_RS01970	423658..425019	-	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
GPY04_RS01980	425623..426600	+	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
GPY04_RS01985	426626..427306	-	681	Protein_365	IS5 family transposase	-
GPY04_RS01990	427648..429168	-	1521	WP_000640055.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T145073 WP_000392003.1 NZ_CP046844:c425019-423658 [Vibrio cholerae C6706]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T145073 NZ_CP062253:5368508-5368762 [Pseudomonas gozinkensis]
ATGAAAATCCAGTTCACGCCGACGGGCTGGGAAGACTACTTGTGGTTTCAGCAAAACGATAAGGCCGGTCTCAAACGAAT
CAATCTTTTGATCAAAGCGATCCAGCGCCAACCCTTTGAAGGCTTGGGTAAACCGGAGCCGCTCAAGCACAACATGAGCG
GCTTCTGGTCACGGCGGATAACTGCCGAGCATCGCCTGGTCTATGCAATCGTAGACGGCGAAATCTGCGTCATAACATGC
AGATTTCACTACTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT145073 WP_000804849.1 NZ_CP046844:c423661-423194 [Vibrio cholerae C6706]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT145073 NZ_CP062253:5368257-5368511 [Pseudomonas gozinkensis]
ATGGACACTATCAACTACACCACAGCTCGTGCGCACTTGGCTGAAACCATGGATCGCGTGAATGAAGACTGCGCCCCGCT
TCTGGTAACCCGCCAAAAAGGCGAGCCTGTAGTGATGATGTCTCTGGCCGAATACAACGCGCTGGAAGAAACGGCCTATC
TGCTGCGTTCTCCGGCCAATGCCGAGCGCTTGATCAAATCGATTGGCGAAATGCGCGCTGGAAAAGTCAAAGTCAGGCAA
CTGATTGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8