Detailed information of TA system TA145053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 819280..821105
• Replicon: chromosome
• Accession: NZ_CP046840
• Organism: Vibrio cholerae strain F9993

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: GPY07_RS03660
• Protein ID: WP_000392003.1
• Coordinates: 819280..820641 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GPY07_RS03665
• Protein ID: WP_000804849.1
• Coordinates: 820638..821105 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY07_RS03640	815131..816651	+	1521	WP_000640055.1	aldehyde dehydrogenase	-
GPY07_RS03645	816993..817673	+	681	Protein_707	IS5 family transposase	-
GPY07_RS03650	817699..818676	-	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
GPY07_RS03660	819280..820641	+	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
GPY07_RS03665	820638..821105	+	468	WP_000804849.1	helix-turn-helix domain-containing protein	Antitoxin
GPY07_RS03670	821533..822111	+	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
GPY07_RS03675	822115..825051	+	2937	WP_001283872.1	DEAD/DEAH box helicase family protein	-
GPY07_RS03680	825121..825825	+	705	WP_001880605.1	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	acfA / acfC / acfB / tcpJ / tcpN/toxT / tcpF / tcpE / tcpT / tcpS / tcpD / tcpR / tcpC / tcpC / tcpQ / tcpB / tcpA / tcpH / tcpP / tcpI	785719..826668	40949
	-	flank	IS/Tn	-	-	817699..818451	752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T145053 WP_000392003.1 NZ_CP046840:819280-820641 [Vibrio cholerae]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T145053 NZ_CP062251:c2749061-2747994 [Pseudomonas sp. IzPS59]
ATGAACCTCGAAAAAATCAAGGGACGCCTCGACTTCCTGCGCGAAGCGGAACGGCTCAAGGACGTCCTCAGAAGCGCGCA
CACGTCCTCCGGTCGCAGCGAAAGCACCGCCGAACACAGCTGGCGTCTGTGCCTGATGGCGATCGTGTTCGCCGACGACC
TCGCCGGGCTCGACATGCTGAAAGTCCTGAAGATGTGCGTCATCCACGACCTCGGCGAGGCCATCAACGGTGACATTCCG
GCGGTCGATCAAGCCGGTTTTCCCGACAAGGGCGAACAGGAGCGCAATGACTTGCTGCTGCTCACCCGCTCTCTGGACGA
CGCGCTGAGAAGCGAAATCCTCGCGCTCTGGGACGACTACGAAAACGCCGGTTCTCCCGAAGCCAAAGCCGTGAAGGCAC
TGGACAAGCTGGAAACACTTTTACAACACACTCAAGGGAAGAATCCGCCAGACTTCGACTATGGCTTCAACCTGGCCTAC
GGCAAGCAATACACCAACGCCGATCCGCTGTTCGAAACCCTTCGGACCCTGATCGACCGGGATACCCGAGAACGCATGAA
TACAAACATCACCGTCCGCAACGAACGCCCCGAAGACATCGACGCCATCGCCCGAATCACCGAGGCCGCGTTCCAGCACG
AAGAACACTCCAGCCACACCGAACAATTCATCGTCAACGCCTTGCGCCGCGCCGGTCAGCTAAGCGTTTCGCTGGTGGCC
GTCGAGAACGACACGGTGGTTGGCCATGTGGCCATTTCACCTGTAACGATTTCCTCCGGCACTCAAGGCTGGTATGGCCT
GGGCCCGATCTCCGTGTGCCCGACCCGCCAGCAGCAAGGCATCGGCTCGGCGCTGATGAAAGACTCGCTGGCGGAGTTGC
AGCGCATCGGCGGCGTTGGCTGCGTGGTGCTTGGCGATCCGGGCTATTACGGTCGTTTCGGCTTCAAGGCCCATGCGGGT
CTGGAGTTGCCGGGGATTCCGGCGGAGTACTTTCAGGCGTTGGCGTTTGGTGGGGAGTTGCCAATCGGTGTCGTCAGCTA
CCACAAGGCCTTTGACGCCACCGAGTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT145053 WP_000804849.1 NZ_CP046840:820638-821105 [Vibrio cholerae]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT145053 NZ_CP062251:c2749704-2749147 [Pseudomonas sp. IzPS59]
ATGCCCGACACCTTGCTCAAGCCCAGTCTCCCCGGCATCGCCCTGCGCCGCTGGCGTGTGCTGCACCGCGTCAAGCAGAG
CCATGCCGCCGAGTTGTTCAAGGTGACCCAGTCGACGATTTCCCGCTGGGAAAGCGGCGTGCAGGCGATGGACCCGGACG
CACACCGACAACTCGAAACGTTGCTCGCGGCGCGCCTCGACAGCGCCGCCGATCAGGCCCTCGCACGACTGGTGACGGAA
AGTGCGCGGCCGGTTCATCTTGTCTGCGACCTGACCCATCGCCTGCTGGCCTGCTCCCGGGCCCGCGCCGCGCAGTTCAG
CAGTCCACTGAGCGATCTGCTGGGGCAATCGCTGTGGCGCTTTGCGACGGCGGAAATCCAGCACAAGGAATCCCTGCTGG
ATGACACCGGATGGCGAGAAGTCCAGGCGCCGGCAGCATTGGAATTTCTGACGGGCAGCAATGACTCCGCCCTCGTGCCG
ATCCAGCCGAGTCTCTGCCGCTGGACGCGGATCGCCCTCTCCGACGGCACCGCCGCACGGCTGGTGGAAACGCTGTAG

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8