Detailed information of TA system TA145052

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2292430..2294152
• Replicon: chromosome
• Accession: NZ_CP046839
• Organism: Vibrio cholerae strain 2011EL-1271

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: GPY01_RS15045
• Protein ID: WP_123161590.1
• Coordinates: 2293214..2294152 (+)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A1U9VYW4
• Locus tag: GPY01_RS15040
• Protein ID: WP_000095578.1
• Coordinates: 2292430..2293221 (+)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY01_RS15005	2287600..2287923	-	324	WP_054103799.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GPY01_RS15010	2288104..2288385	+	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
GPY01_RS15015	2288382..2289008	+	627	WP_000667170.1	hypothetical protein	-
GPY01_RS15020	2288992..2289888	+	897	WP_123161676.1	type-F conjugative transfer system secretin TraK	-
GPY01_RS15025	2289891..2291180	+	1290	WP_123161588.1	TraB/VirB10 family protein	-
GPY01_RS15030	2291255..2291827	+	573	WP_148039504.1	type IV conjugative transfer system lipoprotein TraV	-
GPY01_RS15035	2291824..2292210	+	387	WP_123161589.1	hypothetical protein	-
GPY01_RS15040	2292430..2293221	+	792	WP_000095578.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GPY01_RS15045	2293214..2294152	+	939	WP_123161590.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GPY01_RS15050	2294732..2297461	+	2730	WP_123161591.1	AAA family ATPase	-
GPY01_RS15055	2297458..2298567	+	1110	WP_123161592.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2287600..2301560	13960

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (99-250)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 35612.91 Da
• Isoelectric Point: 4.9081

>T145052 WP_123161590.1 NZ_CP046839:2293214-2294152 [Vibrio cholerae]
MDKDSPYYKQVSLLIRMLPVVATETVFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINTQ
PDIRAAFQDNKADELRIVVSSLVATIKIEVSPVARGTLHSAEIMPVQESVEDEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGISREILVGFLTYTLSHPRPINEVMSPNWQPLNEKFQAEFDGMTFEKVECEDLASVRPMMLIELQK
HFTERDHAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQAKHQEQLDKLKQVLDDWLFNANAE

Nucleotide

• Length: 939 bp

>T145052 NZ_CP062251:c2292904-2292608 [Pseudomonas sp. IzPS59]
ATGCAGGTTGAGTGGCAGCCCGAAGCTCGGGCTGAACTCCTGCACATTCTTGACTACATCAGTGACCGTAATTTCAAAGC
GGCTAACAAGCTCTCAAACGCTATCGAAGCCGCCACGGTTACCCTTACCAAACATCCACACCTCTATCGAGTGGGGCGAA
CTCCCGGCACTCGTGAGATGGTCGTACATCCCAACTACCTGCTCATTTACCAAGCGGTGGGCCGGATAAATATTCTGGGC
GTTGTGCACGCACGTCAGGAATATCCGTCATGTGGCGCTCGGTCACAGAATGAATGA

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 30095.47 Da
• Isoelectric Point: 9.5097

>AT145052 WP_000095578.1 NZ_CP046839:2292430-2293221 [Vibrio cholerae]
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLSSGVYYRPNAQGDIKPTWVDAIQALDVQLGVPVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDAIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRVDETRIKLGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG

Nucleotide

• Length: 792 bp

>AT145052 NZ_CP062251:c2293085-2292894 [Pseudomonas sp. IzPS59]
TTGATCGACCCACTGTGCCCGGTTGTTTCGGATCTTGGTCCCGAGGAGCAGGCTGCGAGTTATGACCGTTGGTTGATCGC
CAAAATACAAGCTTCCATGAATGACCCACGCCCGAGCATTCCTAATGACCAGGTGATGGCCGAAATGCATGCCCTGATGG
AAGCAAAGCGTAAAAAATACGATGCAGGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1U9VYW4