Detailed information of TA system TA145035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 109788..111616
• Replicon: chromosome
• Accession: NZ_CP046835
• Organism: Vibrio vulnificus strain 07-2444

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GPY41_RS07855
• Protein ID: WP_199252198.1
• Coordinates: 110255..111616 (-)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GPY41_RS07850
• Protein ID: WP_158123035.1
• Coordinates: 109788..110270 (-)
• Length: 161 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY41_RS07825	104911..106038	+	1128	WP_199252197.1	ATP-grasp domain-containing protein	-
GPY41_RS07830	106062..106616	+	555	WP_011078504.1	hypothetical protein	-
GPY41_RS07835	106618..107373	+	756	WP_158123033.1	NRDE family protein	-
GPY41_RS07840	107388..107825	+	438	WP_017420004.1	YHS domain-containing protein	-
GPY41_RS07845	107868..109610	+	1743	WP_158123034.1	lysophospholipid acyltransferase family protein	-
GPY41_RS07850	109788..110270	-	483	WP_158123035.1	helix-turn-helix domain-containing protein	Antitoxin
GPY41_RS07855	110255..111616	-	1362	WP_199252198.1	type II toxin-antitoxin system HipA family toxin	Toxin
GPY41_RS07865	112510..112995	-	486	WP_011078466.1	SsrA-binding protein SmpB	-
GPY41_RS07870	113112..113543	+	432	WP_026050324.1	ubiquinone-binding protein	-
GPY41_RS07875	113540..113893	+	354	WP_158123037.1	RnfH family protein	-
GPY41_RS07880	113989..114348	-	360	WP_011078463.1	outer membrane protein assembly factor BamE	-
GPY41_RS07885	114527..116191	-	1665	WP_158123038.1	DNA repair protein RecN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-385)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-139)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51406.20 Da
• Isoelectric Point: 4.7660

>T145035 WP_199252198.1 NZ_CP046835:c111616-110255 [Vibrio vulnificus]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHHDKDDFHAVSLNHPVSFFFDDMGKPGWLKFLDDIM
PSGASRRYWVRYLDIEDLSSDEQDYVLLKFGTMSPVGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYALQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSNHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIA
TDGMRLEEGLNYPSLWLPRFDIQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPVYDFAPMKADPEGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEVLLQELEVTASKCLDLKQRLALRGVPEQILEMPAVGLNHISEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T145035 NZ_CP062250:c2083194-2082943 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 161 a.a.
• Molecular weight: 17570.05 Da
• Isoelectric Point: 10.7497

>AT145035 WP_158123035.1 NZ_CP046835:c110270-109788 [Vibrio vulnificus]
MGATMNKPSNKESVQDTVARLRANRNQHQPKTGTDATHSTKERSQLNTPTRSKTSKNAKAVSAAERKTEANKIIKHLLLG
ELTQGQALKSLRIDILGFKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFGLKVGMLPSSPDVLKSLLIDGEAG

Nucleotide

• Length: 483 bp

>AT145035 NZ_CP062250:c2082946-2082692 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA