Detailed information of TA system TA145017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 743526..744085
• Replicon: chromosome
• Accession: NZ_CP046826
• Organism: Vibrio mimicus strain 07-2442

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D2YI11
• Locus tag: GPY05_RS17385
• Protein ID: WP_000578473.1
• Coordinates: 743526..743804 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GPY05_RS17390
• Protein ID: WP_148535981.1
• Coordinates: 743801..744085 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY05_RS17335	738966..739058	+	93	WP_072935543.1	DUF3265 domain-containing protein	-
GPY05_RS17345	739597..739965	+	369	WP_158163301.1	VOC family protein	-
GPY05_RS17350	740149..740487	+	339	WP_114710012.1	hypothetical protein	-
GPY05_RS17355	740665..741330	+	666	WP_158163302.1	hypothetical protein	-
GPY05_RS17360	741420..741512	+	93	WP_072617168.1	DUF3265 domain-containing protein	-
GPY05_RS17365	741651..741908	+	258	WP_199247023.1	DUF3709 domain-containing protein	-
GPY05_RS17370	742179..742568	+	390	WP_140112821.1	hypothetical protein	-
GPY05_RS17375	742586..742675	+	90	WP_148539388.1	DUF3265 domain-containing protein	-
GPY05_RS17380	742703..743386	+	684	WP_158163303.1	hypothetical protein	-
GPY05_RS17385	743526..743804	-	279	WP_000578473.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
GPY05_RS17390	743801..744085	-	285	WP_148535981.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GPY05_RS17400	744613..744735	+	123	WP_130193147.1	DUF3265 domain-containing protein	-
GPY05_RS17405	744805..745047	+	243	WP_158163304.1	hypothetical protein	-
GPY05_RS17410	745280..745921	+	642	WP_158163305.1	hypothetical protein	-
GPY05_RS17415	745936..746028	+	93	WP_193785314.1	DUF3265 domain-containing protein	-
GPY05_RS17420	746143..747576	+	1434	WP_148503872.1	hypothetical protein	-
GPY05_RS17425	747764..748087	+	324	WP_158163306.1	DUF3709 domain-containing protein	-
GPY05_RS17430	748501..748682	+	182	Protein_660	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	723256..807124	83868
	-	inside	Integron	-	-	724419..798658	74239

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10868.59 Da
• Isoelectric Point: 4.6794

>T145017 WP_000578473.1 NZ_CP046826:c743804-743526 [Vibrio mimicus]
MIFWEEASLNDREKIFEFLYDFNPAAAEKTDELIETKVENLLEQPLMGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Nucleotide

• Length: 279 bp

>T145017 NZ_CP062249:4343924-4344097 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10859.17 Da
• Isoelectric Point: 8.0775

>AT145017 WP_148535981.1 NZ_CP046826:c744085-743801 [Vibrio mimicus]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAAFVEHDIAKAR
MAERKAKIRNRGHA

Nucleotide

• Length: 285 bp

>AT145017 NZ_CP062249:4343600-4343896 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: D2YI11