Detailed information of TA system TA145008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 760456..761015
• Replicon: chromosome
• Accession: NZ_CP046821
• Organism: Vibrio metschnikovii strain 07-2421

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GPX85_RS03405
• Protein ID: WP_158137368.1
• Coordinates: 760737..761015 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GPX85_RS03400
• Protein ID: WP_158137367.1
• Coordinates: 760456..760740 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPX85_RS03365	755758..756294	-	537	WP_158137365.1	hypothetical protein	-
GPX85_RS03370	756291..756482	-	192	WP_158137366.1	hypothetical protein	-
GPX85_RS03375	756580..756690	-	111	WP_158137446.1	DUF3265 domain-containing protein	-
GPX85_RS03380	757909..759255	+	1347	WP_058799734.1	group II intron reverse transcriptase/maturase	-
GPX85_RS03385	759398..759679	-	282	WP_170906768.1	hypothetical protein	-
GPX85_RS03390	759833..760240	-	408	WP_199249642.1	hypothetical protein	-
GPX85_RS03395	760266..760457	-	192	Protein_673	hypothetical protein	-
GPX85_RS03400	760456..760740	+	285	WP_158137367.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GPX85_RS03405	760737..761015	+	279	WP_158137368.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPX85_RS03410	761152..761475	-	324	WP_158137369.1	hypothetical protein	-
GPX85_RS03415	761630..762247	-	618	WP_154170966.1	HAD family phosphatase	-
GPX85_RS03420	762403..763284	-	882	WP_158137370.1	restriction endonuclease	-
GPX85_RS03425	763444..764214	-	771	WP_158137371.1	alpha/beta hydrolase	-
GPX85_RS03430	764390..764998	-	609	WP_158137372.1	DUF3159 domain-containing protein	-
GPX85_RS03435	765030..765140	-	111	WP_158137448.1	DUF3265 domain-containing protein	-
GPX85_RS03440	765675..765797	-	123	WP_158137373.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	725772..821261	95489
	-	inside	Integron	-	katB / cqsA	3..822451	822448
	-	inside	Integron	-	-	735880..767489	31609

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (1-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-75)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10807.54 Da
• Isoelectric Point: 4.6794

>T145008 WP_158137368.1 NZ_CP046821:760737-761015 [Vibrio metschnikovii]
MILWEEASLNDREKIFEFLYDFNPSAAEKTDELIETKVENLLEQPLMGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPAD

Nucleotide

• Length: 279 bp

>T145008 NZ_CP062249:c2084393-2084142 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10888.21 Da
• Isoelectric Point: 9.2198

>AT145008 WP_158137367.1 NZ_CP046821:760456-760740 [Vibrio metschnikovii]
MDTRIQFRVDEEIKRLAQQMAESQGRTLSDACRELTEQLAEQQRKSLSHDAWLTEQVNQAFDKFDSGKAVFVEHNAAKTR
MAERKAKIRNRGHA

Nucleotide

• Length: 285 bp

>AT145008 NZ_CP062249:c2084145-2083891 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA