Detailed information of TA system TA145007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 753335..753897
• Replicon: chromosome
• Accession: NZ_CP046821
• Organism: Vibrio metschnikovii strain 07-2421

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: GPX85_RS03345
• Protein ID: WP_154116302.1
• Coordinates: 753580..753897 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: GPX85_RS03340
• Protein ID: WP_099609954.1
• Coordinates: 753335..753580 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPX85_RS03315	748412..748522	-	111	WP_158137444.1	DUF3265 domain-containing protein	-
GPX85_RS03320	750210..750368	-	159	WP_158137360.1	DUF3265 domain-containing protein	-
GPX85_RS03325	750361..751113	-	753	WP_158137361.1	hypothetical protein	-
GPX85_RS03330	751370..752038	+	669	WP_158137362.1	methyltransferase domain-containing protein	-
GPX85_RS03335	752287..752922	-	636	WP_158137363.1	hypothetical protein	-
GPX85_RS03340	753335..753580	+	246	WP_099609954.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GPX85_RS03345	753580..753897	+	318	WP_154116302.1	CcdB family protein	Toxin
GPX85_RS03350	753905..754027	-	123	WP_158137364.1	DUF3265 domain-containing protein	-
GPX85_RS03355	754496..754912	-	417	WP_158137445.1	DUF1311 domain-containing protein	-
GPX85_RS03360	754942..755052	-	111	WP_081480741.1	DUF3265 domain-containing protein	-
GPX85_RS03365	755758..756294	-	537	WP_158137365.1	hypothetical protein	-
GPX85_RS03370	756291..756482	-	192	WP_158137366.1	hypothetical protein	-
GPX85_RS03375	756580..756690	-	111	WP_158137446.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	725772..821261	95489
	-	inside	Integron	-	katB / cqsA	3..822451	822448
	-	inside	Integron	-	-	735880..767489	31609

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (3-104)

Antitoxin

• Post-segregation antitoxin CcdA (11-81)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11842.64 Da
• Isoelectric Point: 4.6966

>T145007 WP_154116302.1 NZ_CP046821:753580-753897 [Vibrio metschnikovii]
MSQFSLYQNNDKSTSTAYPYFVDVQSELLDTLNTRLVIPLTPVELLDKKAPNHLCPVIHIDQGDFVILTHQMASVPTKIL
REPVNELSTFRNEIIAAIDFLITGI

Nucleotide

• Length: 318 bp

>T145007 NZ_CP062249:c2084145-2083891 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9259.45 Da
• Isoelectric Point: 6.9792

>AT145007 WP_099609954.1 NZ_CP046821:753335-753580 [Vibrio metschnikovii]
MRNSYSTQAPKKATNLTLNSELLAEAKRLNINLSATMEKALEKEVKQRLKTEWLEQNAEAINACNELTENHGLFSDSYRV
F

Nucleotide

• Length: 246 bp

>AT145007 NZ_CP062249:c2084393-2084142 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA