Detailed information of TA system TA144985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/-
• Location: 341483..342261
• Replicon: chromosome
• Accession: NZ_CP046816
• Organism: Vibrio cincinnatiensis strain 1398-82

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A1T4SKI3
• Locus tag: GPX87_RS14525
• Protein ID: WP_039428569.1
• Coordinates: 341483..341746 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: -
• Locus tag: GPX87_RS14530
• Protein ID: WP_078927633.1
• Coordinates: 341746..342261 (+)
• Length: 172 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPX87_RS14495	337074..337925	-	852	WP_158116599.1	IS3 family transposase	-
GPX87_RS14500	338009..338230	-	222	Protein_315	transposase	-
GPX87_RS17445	338729..338890	-	162	WP_199251534.1	DUF3265 domain-containing protein	-
GPX87_RS14510	338928..339341	-	414	WP_115173894.1	hypothetical protein	-
GPX87_RS14515	339368..339523	-	156	WP_115173893.1	DUF3265 domain-containing protein	-
GPX87_RS14520	339722..340099	-	378	WP_078927632.1	hypothetical protein	-
GPX87_RS14525	341483..341746	+	264	WP_039428569.1	DUF4160 domain-containing protein	Toxin
GPX87_RS14530	341746..342261	+	516	WP_078927633.1	DUF2442 domain-containing protein	Antitoxin
GPX87_RS14535	343055..343906	-	852	WP_158116599.1	IS3 family transposase	-
GPX87_RS14540	343990..344211	-	222	Protein_323	transposase	-
GPX87_RS14545	344793..344903	-	111	WP_078927665.1	DUF3265 domain-containing protein	-
GPX87_RS14550	344983..345213	+	231	WP_078927664.1	PAS factor family protein	-
GPX87_RS14555	346289..346399	-	111	WP_080115560.1	DUF3265 domain-containing protein	-
GPX87_RS14560	346455..346745	-	291	WP_115173892.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	242205..347992	105787
	-	inside	Integron	-	-	307705..348744	41039

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (10-75)

Antitoxin

• Uncharacterized protein HI1420 (80-167)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10111.79 Da
• Isoelectric Point: 9.3002

>T144985 WP_039428569.1 NZ_CP046816:341483-341746 [Vibrio cincinnatiensis]
MPEILKKLLGLKFVMYYLDNDSHNRPHVHVEYGEHRASIAIDDIELLAGYLPAKKLVKAEKYIEQHKSELLNEWNKAVKG
VTIKRVN

Nucleotide

• Length: 264 bp

>T144985 NZ_CP062248:c3034183-3033776 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 172 a.a.
• Molecular weight: 19108.41 Da
• Isoelectric Point: 4.2518

>AT144985 WP_078927633.1 NZ_CP046816:341746-342261 [Vibrio cincinnatiensis]
MLKIIDVDWESGYELELEFSDGFRGIADLTAIFETEAFSQVDSFTEFSLEGTYIDWGAAEISATKLRQLAEEQGKYLEAK
ERAISPDDIEAVLKQAAWDSITLNRPDILQAAIRGYIEEYGVQNVQLKTNLKSRPSIYKALSPHTSPKFDTLVQLAHGAL
AVKAENEAFSQ

Nucleotide

• Length: 516 bp

>AT144985 NZ_CP062248:c3034430-3034164 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A1T4SKI3