Detailed information of TA system TA144984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 326901..327472
• Replicon: chromosome
• Accession: NZ_CP046816
• Organism: Vibrio cincinnatiensis strain 1398-82

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A1T4SJW3
• Locus tag: GPX87_RS14415
• Protein ID: WP_000351249.1
• Coordinates: 326901..327302 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q8GNG3
• Locus tag: GPX87_RS14420
• Protein ID: WP_001080654.1
• Coordinates: 327302..327472 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPX87_RS14375	322133..322249	-	117	WP_113594551.1	DUF3265 domain-containing protein	-
GPX87_RS14380	322246..322548	-	303	WP_113594550.1	hypothetical protein	-
GPX87_RS14385	322549..322704	-	156	WP_158142139.1	hypothetical protein	-
GPX87_RS14390	322865..323284	-	420	WP_025590728.1	hypothetical protein	-
GPX87_RS14395	323286..323492	-	207	WP_115173896.1	transposase	-
GPX87_RS14400	324683..325342	-	660	WP_038970004.1	hypothetical protein	-
GPX87_RS14405	325478..325993	-	516	WP_078927623.1	peptide deformylase	-
GPX87_RS14410	326148..326744	-	597	WP_078927624.1	glutathione S-transferase family protein	-
GPX87_RS14415	326901..327302	-	402	WP_000351249.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GPX87_RS14420	327302..327472	-	171	WP_001080654.1	hypothetical protein	Antitoxin
GPX87_RS14425	327543..327668	-	126	WP_115173939.1	DUF3265 domain-containing protein	-
GPX87_RS14430	327650..328636	-	987	WP_078927625.1	DUF3644 domain-containing protein	-
GPX87_RS14435	328702..329553	-	852	WP_158142140.1	IS3 family transposase	-
GPX87_RS14440	329637..329858	-	222	Protein_304	transposase	-
GPX87_RS14445	330944..331054	-	111	Protein_305	DUF3265 domain-containing protein	-
GPX87_RS14450	331051..331410	-	360	WP_001071517.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	242205..347992	105787
	-	inside	Integron	-	-	307705..348744	41039

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14636.77 Da
• Isoelectric Point: 4.0128

>T144984 WP_000351249.1 NZ_CP046816:c327302-326901 [Vibrio cincinnatiensis]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSVL

Nucleotide

• Length: 402 bp

>T144984 NZ_CP062248:c2904451-2904116 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6287.35 Da
• Isoelectric Point: 10.7274

>AT144984 WP_001080654.1 NZ_CP046816:c327472-327302 [Vibrio cincinnatiensis]
MNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 171 bp

>AT144984 NZ_CP062248:c2904699-2904451 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A1T4SJW3

Antitoxin

• AlphaFold DB: A0A1T4SJJ1