Detailed information of TA system TA144983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 309435..310201
• Replicon: chromosome
• Accession: NZ_CP046816
• Organism: Vibrio cincinnatiensis strain 1398-82

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GPX87_RS14290
• Protein ID: WP_078927600.1
• Coordinates: 309704..310201 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: C9P137
• Locus tag: GPX87_RS14285
• Protein ID: WP_004394087.1
• Coordinates: 309435..309707 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPX87_RS14245	304438..305763	-	1326	WP_115173883.1	group II intron reverse transcriptase/maturase	-
GPX87_RS14250	306274..306630	-	357	Protein_267	DUF4102 domain-containing protein	-
GPX87_RS14255	306906..307715	-	810	WP_078927605.1	hypothetical protein	-
GPX87_RS14260	307705..308148	-	444	WP_078927604.1	hypothetical protein	-
GPX87_RS14265	308176..308301	-	126	WP_078927603.1	DUF3265 domain-containing protein	-
GPX87_RS14270	308283..308714	-	432	WP_078927602.1	hypothetical protein	-
GPX87_RS14275	308869..309192	-	324	WP_078927601.1	hypothetical protein	-
GPX87_RS14280	309234..309395	-	162	WP_172459867.1	DUF3265 domain-containing protein	-
GPX87_RS14285	309435..309707	+	273	WP_004394087.1	DUF1778 domain-containing protein	Antitoxin
GPX87_RS14290	309704..310201	+	498	WP_078927600.1	GNAT family N-acetyltransferase	Toxin
GPX87_RS14300	310311..311144	-	834	WP_115173899.1	hypothetical protein	-
GPX87_RS14305	311144..311989	-	846	WP_078927599.1	pentapeptide repeat-containing protein	-
GPX87_RS14310	312161..313135	-	975	WP_078927598.1	FRG domain-containing protein	-
GPX87_RS14315	313184..314035	-	852	WP_158116599.1	IS3 family transposase	-
GPX87_RS14320	314119..314340	-	222	Protein_280	transposase	-
GPX87_RS14325	314422..314559	-	138	Protein_281	DUF3265 domain-containing protein	-
GPX87_RS14330	314540..314953	-	414	WP_115173898.1	GFA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	242205..347992	105787
	-	inside	Integron	-	-	307705..348744	41039

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18569.42 Da
• Isoelectric Point: 9.5686

>T144983 WP_078927600.1 NZ_CP046816:309704-310201 [Vibrio cincinnatiensis]
MMKTVLLDKDKHDRNRFNCGIDALNNYLKVMASQQAKKDNTRTFVLEDENNTSLIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAIDERYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKLFYERYGFKSFQDAENKLFITISDI
RASLG

Nucleotide

• Length: 498 bp

>T144983 NZ_CP062248:2770813-2771142 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9872.43 Da
• Isoelectric Point: 9.7681

>AT144983 WP_004394087.1 NZ_CP046816:309435-309707 [Vibrio cincinnatiensis]
MATTLPRITARVDVDTQNLLTKAAALAGMSSINSFVLNAAIEKAKQVIEREQTLKLSQADAILLMEALDKPTTLNQKLKT
AAERYETKNQ

Nucleotide

• Length: 273 bp

>AT144983 NZ_CP062248:2770475-2770792 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A1T4SI33