Detailed information of TA system TA144977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 71976..72742
• Replicon: chromosome
• Accession: NZ_CP046812
• Organism: Vibrio alginolyticus strain 2010V-1102

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GPY28_RS00385
• Protein ID: WP_053307291.1
• Coordinates: 71976..72473 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A3N3DV16
• Locus tag: GPY28_RS00390
• Protein ID: WP_013571703.1
• Coordinates: 72470..72742 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY28_RS00350	67433..68194	-	762	WP_053307370.1	hypothetical protein	-
GPY28_RS00355	68197..68736	-	540	WP_053307371.1	hypothetical protein	-
GPY28_RS00360	69581..70216	+	636	WP_053307288.1	hypothetical protein	-
GPY28_RS00365	70459..70749	-	291	WP_053307289.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GPY28_RS00370	70739..70987	-	249	WP_005377003.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GPY28_RS00375	71062..71154	+	93	WP_196334432.1	DUF3265 domain-containing protein	-
GPY28_RS00380	71185..71832	+	648	WP_053307290.1	DUF4263 domain-containing protein	-
GPY28_RS00385	71976..72473	-	498	WP_053307291.1	GNAT family N-acetyltransferase	Toxin
GPY28_RS00390	72470..72742	-	273	WP_013571703.1	DUF1778 domain-containing protein	Antitoxin
GPY28_RS00395	72976..73428	+	453	WP_053307292.1	hypothetical protein	-
GPY28_RS00400	73548..73826	-	279	WP_053307293.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
GPY28_RS00405	73823..74107	-	285	WP_053307294.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
GPY28_RS00410	74184..74276	+	93	WP_196334436.1	DUF3265 domain-containing protein	-
GPY28_RS00415	74797..74886	+	90	WP_080996190.1	DUF3265 domain-containing protein	-
GPY28_RS00420	75252..75731	+	480	WP_053307295.1	DUF1456 family protein	-
GPY28_RS00430	76477..77199	-	723	WP_199266780.1	Crp/Fnr family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18474.12 Da
• Isoelectric Point: 8.8085

>T144977 WP_053307291.1 NZ_CP046812:c72473-71976 [Vibrio alginolyticus]
MMNTVLLDKAKHDRNRFNCGIEALNNYLKVMASQQAKKDNTRTFVLEDDSDNSHVIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITISDV
RASLG

Nucleotide

• Length: 498 bp

>T144977 NZ_CP062248:c1639891-1639604 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9747.28 Da
• Isoelectric Point: 8.5960

>AT144977 WP_013571703.1 NZ_CP046812:c72742-72470 [Vibrio alginolyticus]
MATTLPRITARVDVDTQDLLTKAAAIAGMSSINSFVLSAAIEKAKQVIEREQALKLSQADAMLLMEALDRPATQNSKLKA
AADRYESKIQ

Nucleotide

• Length: 273 bp

>AT144977 NZ_CP062248:c1640130-1639891 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3N3DV16