Detailed information of TA system TA144954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 547746..548274
• Replicon: chromosome
• Accession: NZ_CP046801
• Organism: Vibrio cincinnatiensis strain 2070-81

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0F2IAX4
• Locus tag: GPX92_RS02525
• Protein ID: WP_010320326.1
• Coordinates: 547746..548033 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W9V7T8
• Locus tag: GPX92_RS02530
• Protein ID: WP_001250181.1
• Coordinates: 548023..548274 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPX92_RS02470	543266..543382	-	117	WP_094501664.1	DUF3265 domain-containing protein	-
GPX92_RS02475	543373..543657	-	285	WP_094501666.1	antibiotic biosynthesis monooxygenase	-
GPX92_RS17555	543590..543799	-	210	WP_202981980.1	DUF3265 domain-containing protein	-
GPX92_RS02485	543837..544250	-	414	WP_115173894.1	hypothetical protein	-
GPX92_RS02490	544765..544875	-	111	WP_072668974.1	DUF3265 domain-containing protein	-
GPX92_RS02495	544872..545708	-	837	WP_158116636.1	TIGR02391 family protein	-
GPX92_RS02500	545852..546082	-	231	WP_158116637.1	hypothetical protein	-
GPX92_RS02505	546095..546655	-	561	WP_158116638.1	hypothetical protein	-
GPX92_RS02510	546683..546793	-	111	Protein_498	DUF3265 domain-containing protein	-
GPX92_RS02515	547184..547483	-	300	WP_078927646.1	hypothetical protein	-
GPX92_RS02520	547631..547741	-	111	WP_158116801.1	DUF3265 domain-containing protein	-
GPX92_RS02525	547746..548033	-	288	WP_010320326.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPX92_RS02530	548023..548274	-	252	WP_001250181.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GPX92_RS02535	548481..548867	-	387	WP_158116639.1	VOC family protein	-
GPX92_RS02540	548935..549786	-	852	WP_158116599.1	IS3 family transposase	-
GPX92_RS02545	549870..550091	-	222	Protein_505	transposase	-
GPX92_RS02550	551050..552183	-	1134	WP_078927662.1	ISAs1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	551050..552183	1133
	-	inside	Integron	-	-	536519..550286	13767

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (11-62)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11038.97 Da
• Isoelectric Point: 10.5827

>T144954 WP_010320326.1 NZ_CP046801:c548033-547746 [Vibrio cincinnatiensis]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSSKLRGYDSVYKIKLRTAGYRLAYEVIDNEVVVYVLAVGK
RDKDAVYKKLASRFS

Nucleotide

• Length: 288 bp

>T144954 NZ_CP062247:c2084397-2084146 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9170.32 Da
• Isoelectric Point: 4.0197

>AT144954 WP_001250181.1 NZ_CP046801:c548274-548023 [Vibrio cincinnatiensis]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLMDMLDDYELSQIVDSRRGDLSQAVEINI
DDL

Nucleotide

• Length: 252 bp

>AT144954 NZ_CP062247:c2084149-2083895 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A0F2IAX4

Antitoxin

• AlphaFold DB: W9V7T8