Detailed information of TA system TA144947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1035479..1037307
• Replicon: chromosome
• Accession: NZ_CP046793
• Organism: Vibrio metschnikovii strain 9502-00

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GPX86_RS04630
• Protein ID: WP_158144163.1
• Coordinates: 1035479..1036840 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GPX86_RS04635
• Protein ID: WP_158144164.1
• Coordinates: 1036825..1037307 (+)
• Length: 161 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPX86_RS04600	1032173..1032532	+	360	WP_040904610.1	outer membrane protein assembly factor BamE	-
GPX86_RS04605	1032672..1032995	-	324	WP_040904612.1	RnfH family protein	-
GPX86_RS04610	1032992..1033426	-	435	WP_004395789.1	ubiquinone-binding protein	-
GPX86_RS04615	1033708..1034193	+	486	WP_004395790.1	SsrA-binding protein SmpB	-
GPX86_RS04625	1034794..1035243	+	450	Protein_909	integrase arm-type DNA-binding domain-containing protein	-
GPX86_RS04630	1035479..1036840	+	1362	WP_158144163.1	type II toxin-antitoxin system HipA family toxin	Toxin
GPX86_RS04635	1036825..1037307	+	483	WP_158144164.1	helix-turn-helix domain-containing protein	Antitoxin
GPX86_RS04640	1037629..1038039	-	411	WP_004395791.1	VOC family protein	-
GPX86_RS04645	1038155..1038601	+	447	WP_158144165.1	redox-sensitive transcriptional activator SoxR	-
GPX86_RS04650	1038754..1039548	-	795	WP_158144166.1	MetQ/NlpA family ABC transporter substrate-binding protein	-
GPX86_RS04655	1039698..1040045	+	348	WP_154168894.1	carboxymuconolactone decarboxylase family protein	-
GPX86_RS04660	1040150..1041139	-	990	WP_158138006.1	sulfate ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-385)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-139)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51446.35 Da
• Isoelectric Point: 5.0619

>T144947 WP_158144163.1 NZ_CP046793:1035479-1036840 [Vibrio metschnikovii]
MEEITVQAFIKDEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHHDKDDFHAVSLNHPVSFFFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSNHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIA
TEGMRLEEGLNYPSLWLPRFDVQIINQQIKRFGMESVYSILKKGAGVTLDHETTIRTLIEKITESHMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPVYDFAPMKADPEGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEVLLQELEVTASKCIDLKQRLALRGVPEQILEMPAVGLNHISEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T144947 NZ_CP062247:c1409387-1409193 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 161 a.a.
• Molecular weight: 17542.05 Da
• Isoelectric Point: 10.9994

>AT144947 WP_158144164.1 NZ_CP046793:1036825-1037307 [Vibrio metschnikovii]
MGATMNKPSNKESVQDTVARLRANRNQHQPNTGTNATHSTKERSQLHTPTRSKTSKNAKAVSAAERKTEANKIIKHLLLG
ELTQGQALKSLRINILGLKQDVFARLVNVSRKTLSDIENDRGSYNTEILNKVFKPFGLKVGMLPSSPDVLKSLLIDGEAG

Nucleotide

• Length: 483 bp

>AT144947 NZ_CP062247:1409016-1409194 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT