Detailed information of TA system TA144921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafN(antitoxin)
• Location: 76049..76614
• Replicon: plasmid unnamed1
• Accession: NZ_CP046774
• Organism: Vibrio alginolyticus strain 2014V-1011

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GPY59_RS25575
• Protein ID: WP_158174297.1
• Coordinates: 76049..76336 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U3BLE1
• Locus tag: GPY59_RS25580
• Protein ID: WP_000086647.1
• Coordinates: 76333..76614 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY59_RS25555	71248..72531	+	1284	WP_033906201.1	TolC family protein	-
GPY59_RS25560	72528..74654	+	2127	WP_050550079.1	type I secretion system permease/ATPase	-
GPY59_RS25565	74633..75790	+	1158	WP_154204499.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GPY59_RS25570	75929..76021	-	93	WP_199266772.1	DUF3265 domain-containing protein	-
GPY59_RS25575	76049..76336	-	288	WP_158174297.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPY59_RS25580	76333..76614	-	282	WP_000086647.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GPY59_RS25585	76827..77273	-	447	WP_158174298.1	GNAT family N-acetyltransferase	-
GPY59_RS25590	77336..77695	-	360	WP_158174360.1	HNH endonuclease	-
GPY59_RS26175	78028..78115	-	88	Protein_62	DUF3265 domain-containing protein	-
GPY59_RS25595	78144..78644	-	501	WP_158174299.1	hypothetical protein	-
GPY59_RS26180	79235..79327	-	93	WP_079858050.1	DUF3265 domain-containing protein	-
GPY59_RS25605	79397..79675	-	279	WP_158174301.1	hypothetical protein	-
GPY59_RS25610	79828..80280	-	453	WP_158174302.1	hypothetical protein	-
GPY59_RS26185	80309..80401	-	93	WP_005448238.1	DUF3265 domain-containing protein	-
GPY59_RS25620	80970..81332	-	363	WP_158174304.1	hypothetical protein	-
GPY59_RS25625	81342..81434	-	93	WP_199266773.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..137319	137319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-74)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11046.76 Da
• Isoelectric Point: 5.6096

>T144921 WP_158174297.1 NZ_CP046774:c76336-76049 [Vibrio alginolyticus]
MKVVWSPLALQKLGDAAEFISLDNPLAAERWVNEVFDKTELLGSMPEMGRFVPEMPHTNYREIIFGHYRIIYSLSHEIRV
LTVRNCRQILSENDV

Nucleotide

• Length: 288 bp

>T144921 NZ_CP062246:1503543-1503719 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10398.91 Da
• Isoelectric Point: 5.8650

>AT144921 WP_000086647.1 NZ_CP046774:c76614-76333 [Vibrio alginolyticus]
MSRIHFDQDIQPLSEFRAGVASYIKQINETRRPLVITQRGKGVAVVLDVAEYEAMQEKIELLEEMRTAEAQLASGLGVSN
DDARAQVLGRIKK

Nucleotide

• Length: 282 bp

>AT144921 NZ_CP062246:1503765-1504181 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4P8G0X0