Detailed information of TA system TA144900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 5599..6139
• Replicon: chromosome
• Accession: NZ_CP046750
• Organism: Vibrio cholerae strain 2015V-1118

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A151JGK8
• Locus tag: GPX93_RS13485
• Protein ID: WP_000277238.1
• Coordinates: 5870..6139 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9KML3
• Locus tag: GPX93_RS13480
• Protein ID: WP_001258569.1
• Coordinates: 5599..5877 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPX93_RS13450	1396..1434	-	39	WP_113773576.1	hypothetical protein	-
GPX93_RS13455	1450..1575	-	126	WP_123161839.1	DUF645 family protein	-
GPX93_RS13460	1881..2798	-	918	WP_123161840.1	alpha/beta hydrolase	-
GPX93_RS13465	3534..3941	-	408	WP_032473180.1	DUF1398 family protein	-
GPX93_RS13470	4158..4341	-	184	Protein_7	DUF645 family protein	-
GPX93_RS13475	4634..5347	-	714	WP_000123659.1	Fic family protein	-
GPX93_RS13480	5599..5877	+	279	WP_001258569.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GPX93_RS13485	5870..6139	+	270	WP_000277238.1	type II toxin-antitoxin system YafQ family toxin	Toxin
GPX93_RS13490	6336..6515	-	180	WP_080475853.1	DUF645 family protein	-
GPX93_RS13495	6706..6834	-	129	Protein_12	DUF3265 domain-containing protein	-
GPX93_RS13500	6841..7260	-	420	WP_199249011.1	TIR domain-containing protein	-
GPX93_RS13505	7402..7791	-	390	WP_000491261.1	VOC family protein	-
GPX93_RS13515	7995..8513	-	519	WP_057556356.1	GNAT family N-acetyltransferase	-
GPX93_RS13520	8716..8961	+	246	WP_001260801.1	type II toxin-antitoxin system CcdA family antitoxin	-
GPX93_RS13525	8961..9278	+	318	WP_123161843.1	CcdB family protein	-
GPX93_RS13535	9396..9911	-	516	WP_000343786.1	GNAT family N-acetyltransferase	-
GPX93_RS13540	10046..10708	-	663	WP_123161845.1	Vat family streptogramin A O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	hlyA / vgrG-3 / vasL / icmF/vasK / vasJ / vasI / vasH / clpB/vasG / vasF / vasE / vasD / vasC / vasB / vasA / VCA0109 / vipB/mglB / vipA/mglA / vgrG-2 / hcp-2 / hlyA / htpB / ugd / cqsA	2..1102730	1102728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10406.11 Da
• Isoelectric Point: 9.5084

>T144900 WP_000277238.1 NZ_CP046750:5870-6139 [Vibrio cholerae]
MYKLEYSTQFKKDFKKITKMPISDIIEVGNVISKLQRGEKLEPKNVDHPLTGNWVGFRDCHIKPDLVLIYRVFNDQLQLA
RIGSHSDLF

Nucleotide

• Length: 270 bp

>T144900 NZ_CP062245:c2083193-2082942 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10088.58 Da
• Isoelectric Point: 6.0728

>AT144900 WP_001258569.1 NZ_CP046750:5599-5877 [Vibrio cholerae]
MRTEMLSTRIDHDTKIAFTNVCDEMGLSTSQAIKLFAKAVINHGGIPFELRVPQPNEVTASAIQELVEGKGHKAESVEAM
LNELTEGKVKHV

Nucleotide

• Length: 279 bp

>AT144900 NZ_CP062245:c2082945-2082691 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A151JGK8

Antitoxin

• AlphaFold DB: A0A151KTP9