Detailed information of TA system TA144899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 694863..696688
• Replicon: chromosome
• Accession: NZ_CP046749
• Organism: Vibrio cholerae strain 2015V-1118

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GPX93_RS03245
• Protein ID: WP_184482644.1
• Coordinates: 695327..696688 (-)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GPX93_RS03240
• Protein ID: WP_032472658.1
• Coordinates: 694863..695330 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPX93_RS03230	690932..693853	-	2922	WP_123162463.1	DEAD/DEAH box helicase family protein	-
GPX93_RS03235	693857..694435	-	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
GPX93_RS03240	694863..695330	-	468	WP_032472658.1	helix-turn-helix domain-containing protein	Antitoxin
GPX93_RS03245	695327..696688	-	1362	WP_184482644.1	type II toxin-antitoxin system HipA family toxin	Toxin
GPX93_RS03255	697174..698343	-	1170	WP_000100558.1	hypothetical protein	-
GPX93_RS03260	698851..699486	+	636	WP_123162465.1	inovirus Gp2 family protein	-
GPX93_RS03265	699551..700219	+	669	WP_123162466.1	inovirus Gp2 family protein	-
GPX93_RS03270	700280..700876	+	597	WP_123162467.1	inovirus Gp2 family protein	-
GPX93_RS03275	700941..701141	+	201	WP_001211744.1	AlpA family transcriptional regulator	-
GPX93_RS03280	701224..701670	+	447	WP_053037330.1	ribonuclease H	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51314.29 Da
• Isoelectric Point: 4.9785

>T144899 WP_184482644.1 NZ_CP046749:c696688-695327 [Vibrio cholerae]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALKHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHESGSEKIWIDPYQDDNSNHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLAKWGLL

Nucleotide

• Length: 1362 bp

>T144899 NZ_CP062245:c2082945-2082691 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17073.60 Da
• Isoelectric Point: 10.7084

>AT144899 WP_032472658.1 NZ_CP046749:c695330-694863 [Vibrio cholerae]
MKPSNKESVQDTVARLRANRNQQQPKVGKNATHSIKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT144899 NZ_CP062245:c2083193-2082942 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA