Detailed information of TA system TA144897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 661691..662262
• Replicon: chromosome
• Accession: NZ_CP046748
• Organism: Vibrio cholerae strain 3541-04

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9KMA2
• Locus tag: GPY00_RS17355
• Protein ID: WP_000351248.1
• Coordinates: 661691..662092 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q8GNG3
• Locus tag: GPY00_RS17360
• Protein ID: WP_001080654.1
• Coordinates: 662092..662262 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY00_RS17310	656897..657235	-	339	WP_019829637.1	hypothetical protein	-
GPY00_RS17320	657896..658075	-	180	WP_044128174.1	DUF645 family protein	-
GPY00_RS17325	658390..659028	-	639	WP_019829928.1	hypothetical protein	-
GPY00_RS17330	659164..659742	-	579	WP_001020574.1	DJ-1/PfpI family protein	-
GPY00_RS17335	660074..660247	-	174	WP_080285841.1	DUF3709 domain-containing protein	-
GPY00_RS17340	660713..660865	-	153	WP_199252431.1	DUF3709 domain-containing protein	-
GPY00_RS17345	661212..661394	-	183	WP_199252432.1	DUF645 family protein	-
GPY00_RS17350	661578..661721	-	144	WP_199252451.1	DUF3265 domain-containing protein	-
GPY00_RS17355	661691..662092	-	402	WP_000351248.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GPY00_RS17360	662092..662262	-	171	WP_001080654.1	hypothetical protein	Antitoxin
GPY00_RS17365	662502..662750	+	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GPY00_RS17370	662740..663030	+	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GPY00_RS17375	663048..663158	-	111	Protein_622	DUF3265 domain-containing protein	-
GPY00_RS17380	663155..663913	-	759	WP_055050422.1	hypothetical protein	-
GPY00_RS17385	664059..664415	-	357	WP_033838071.1	hypothetical protein	-
GPY00_RS17390	664573..665220	-	648	WP_044128203.1	glutathione S-transferase family protein	-
GPY00_RS17395	665398..665640	-	243	WP_053034790.1	hypothetical protein	-
GPY00_RS17405	665840..666580	-	741	WP_161779866.1	PhzF family phenazine biosynthesis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	597132..672561	75429
	-	inside	Integron	-	-	597132..668045	70913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-132)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14608.71 Da
• Isoelectric Point: 4.0128

>T144897 WP_000351248.1 NZ_CP046748:c662092-661691 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Nucleotide

• Length: 402 bp

>T144897 NZ_CP062245:c1917463-1917360 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6287.35 Da
• Isoelectric Point: 10.7274

>AT144897 WP_001080654.1 NZ_CP046748:c662262-662092 [Vibrio cholerae]
MNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 171 bp

>AT144897 NZ_CP062245:1917322-1917593 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0Q0PN34

Antitoxin

• AlphaFold DB: A0A1T4SJJ1