Detailed information of TA system TA144896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 631855..632393
• Replicon: chromosome
• Accession: NZ_CP046748
• Organism: Vibrio cholerae strain 3541-04

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q9KMJ0
• Locus tag: GPY00_RS17070
• Protein ID: WP_000802136.1
• Coordinates: 632094..632393 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A271VK51
• Locus tag: GPY00_RS17065
• Protein ID: WP_001107720.1
• Coordinates: 631855..632097 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY00_RS17030	627498..627599	-	102	Protein_562	DUF645 family protein	-
GPY00_RS17035	627928..628323	-	396	WP_044128232.1	DUF3465 domain-containing protein	-
GPY00_RS17040	628489..629205	-	717	WP_044128153.1	class I SAM-dependent methyltransferase	-
GPY00_RS17045	629389..629805	-	417	WP_019830302.1	DUF1311 domain-containing protein	-
GPY00_RS17050	629956..630435	-	480	WP_032473214.1	hypothetical protein	-
GPY00_RS17055	630571..631005	-	435	WP_044128155.1	GNAT family N-acetyltransferase	-
GPY00_RS17060	631163..631645	-	483	WP_032473215.1	tetratricopeptide repeat protein	-
GPY00_RS17065	631855..632097	+	243	WP_001107720.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
GPY00_RS17070	632094..632393	+	300	WP_000802136.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPY00_RS17075	632401..632511	-	111	WP_072599357.1	DUF3265 domain-containing protein	-
GPY00_RS17080	632508..632861	-	354	WP_019829948.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
GPY00_RS19185	633060..633230	-	171	WP_000483444.1	DUF645 family protein	-
GPY00_RS17090	633549..633974	-	426	WP_025987830.1	hypothetical protein	-
GPY00_RS19190	634204..634866	-	663	WP_000084911.1	Vat family streptogramin A O-acetyltransferase	-
GPY00_RS17100	635084..636043	+	960	WP_113592640.1	IS30 family transposase	-
GPY00_RS17105	636066..636425	-	360	WP_019830329.1	VOC family protein	-
GPY00_RS19195	636635..636809	-	175	Protein_578	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	597132..672561	75429
	-	inside	Integron	-	-	597132..668045	70913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11589.29 Da
• Isoelectric Point: 9.9232

>T144896 WP_000802136.1 NZ_CP046748:632094-632393 [Vibrio cholerae]
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKFPQGSHVIFYQQTGSQQ
IRVIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T144896 NZ_CP062245:c1639889-1639602 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.82 Da
• Isoelectric Point: 4.1677

>AT144896 WP_001107720.1 NZ_CP046748:631855-632097 [Vibrio cholerae]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLVEGEQSGDADYDLDSFINELDSENIR

Nucleotide

• Length: 243 bp

>AT144896 NZ_CP062245:c1640128-1639889 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 7R5A

Antitoxin

• AlphaFold DB: A0A271VK51