Detailed information of TA system TA144894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 612054..612820
• Replicon: chromosome
• Accession: NZ_CP046748
• Organism: Vibrio cholerae strain 3541-04

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: GPY00_RS16900
• Protein ID: WP_000982260.1
• Coordinates: 612323..612820 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GPY00_RS16895
• Protein ID: WP_019829878.1
• Coordinates: 612054..612326 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY00_RS16850	607302..607718	-	417	WP_158128766.1	hypothetical protein	-
GPY00_RS16860	607883..608536	-	654	WP_019829910.1	peptidoglycan DD-metalloendopeptidase family protein	-
GPY00_RS16870	608686..609264	-	579	WP_001020574.1	DJ-1/PfpI family protein	-
GPY00_RS16875	609418..610056	-	639	WP_019829928.1	hypothetical protein	-
GPY00_RS16880	610191..610688	-	498	WP_005526565.1	GNAT family N-acetyltransferase	-
GPY00_RS16885	610893..611063	-	171	WP_000483444.1	DUF645 family protein	-
GPY00_RS16890	611403..611807	-	405	WP_019829940.1	hypothetical protein	-
GPY00_RS16895	612054..612326	+	273	WP_019829878.1	DUF1778 domain-containing protein	Antitoxin
GPY00_RS16900	612323..612820	+	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
GPY00_RS16905	612955..613914	-	960	WP_044128144.1	restriction endonuclease	-
GPY00_RS16910	614501..614956	-	456	WP_001245329.1	GNAT family N-acetyltransferase	-
GPY00_RS16915	615083..615514	-	432	WP_044128141.1	hypothetical protein	-
GPY00_RS16920	615655..616428	-	774	WP_139284613.1	hypothetical protein	-
GPY00_RS16925	616562..617046	-	485	Protein_544	DUF1993 family protein	-
GPY00_RS16930	617204..617593	-	390	WP_000491261.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	597132..672561	75429
	-	inside	Integron	-	-	597132..668045	70913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-89)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T144894 WP_000982260.1 NZ_CP046748:612323-612820 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T144894 NZ_CP062245:1503542-1503718 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9713.24 Da
• Isoelectric Point: 6.2988

>AT144894 WP_019829878.1 NZ_CP046748:612054-612326 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQTDAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT144894 NZ_CP062245:1503764-1504180 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1