Detailed information of TA system TA144893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PanAT/DUF4065(antitoxin)
• Location: 1489733..1491408
• Replicon: chromosome
• Accession: NZ_CP046747
• Organism: Vibrio cholerae strain 3541-04

Toxin (Protein)

• Gene name: panT
• Uniprot ID: -
• Locus tag: GPY00_RS06990
• Protein ID: WP_158128524.1
• Coordinates: 1490197..1491408 (+)
• Length: 404 a.a.

Antitoxin (Protein)

• Gene name: panA
• Uniprot ID: A0A0X8LPP8
• Locus tag: GPY00_RS06985
• Protein ID: WP_000649312.1
• Coordinates: 1489733..1490200 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY00_RS06950	1485144..1485320	-	177	WP_158128519.1	hypothetical protein	-
GPY00_RS06955	1485324..1485767	-	444	WP_158128520.1	DUF2787 domain-containing protein	-
GPY00_RS06960	1485818..1486255	-	438	WP_199252422.1	DUF2787 domain-containing protein	-
GPY00_RS06965	1486252..1486725	-	474	WP_148528079.1	DNA repair protein RadC	-
GPY00_RS06970	1486941..1487078	+	138	WP_158128522.1	hypothetical protein	-
GPY00_RS06975	1487142..1488779	+	1638	WP_158128523.1	RNA-directed DNA polymerase	-
GPY00_RS06980	1488806..1489513	+	708	WP_123946169.1	hypothetical protein	-
GPY00_RS06985	1489733..1490200	+	468	WP_000649312.1	DUF4065 domain-containing protein	Antitoxin
GPY00_RS06990	1490197..1491408	+	1212	WP_158128524.1	RelA/SpoT domain-containing protein	Toxin
GPY00_RS06995	1491654..1492763	-	1110	WP_001888924.1	tRNA (uridine(54)-C5)-methyltransferase TrmA	-
GPY00_RS07000	1493161..1494993	+	1833	WP_044128127.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1469910..1491408	21498

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (111-228)

Antitoxin

• Antitoxin SocA-like, Panacea domain (32-123)

Sequences

Toxin

Protein

• Length: 404 a.a.
• Molecular weight: 45796.67 Da
• Isoelectric Point: 5.2761

>T144893 WP_158128524.1 NZ_CP046747:1490197-1491408 [Vibrio cholerae]
VSDKEKRVVSFQIDDTPTAEQASKNFQDDYELCFQDFLERTVDESAAEDFSYSNNGVKKAGRRLRKKEGDLKSATATIQQ
FRAAHEKPLNTIAYLVGRCCRELGIDVKPVTRLKRLETIVDKLQRKSLDGETTNATCVTNMNDIGGCRAIFPDLESLEKA
KERLQVTVEKVGRIKIKDIDDYITNPKPNDCGYRSLHVIYRYDHASGKSFNIEAQLRTRLQHLWATTVEIVDILEGTKIK
THSHSPDEDKEKLQVQWEELLSIMSQYIADAEGAIELSCDNRAQFSNRLIELNREINALSRLRSFKILSEKVEGCRDGSI
EHVLLVIDETSLEMIFSEEFENYTQAISVYNAAEKMTRSHARLNTLLISTKNMGQLSEAYPNYLGDCASFITLLEDAMQS
GQN

Nucleotide

• Length: 1212 bp

>T144893 NZ_CP062245:c1408183-1407989 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17581.02 Da
• Isoelectric Point: 7.5659

>AT144893 WP_000649312.1 NZ_CP046747:1489733-1490200 [Vibrio cholerae]
MKAYHTYSAVDVAWSLLKHAKEQGKCFSNLQLQKLTYVCHGLSLAHFQRPLVVDDVFAWKFGPVVPSVYFRFKSYGSNVI
TEQCDVVLDHESESIVKDVVSQLGHLTGPQLVDLTHREGSPWHQVWDGTHQKVIPDHLIQAHYTQIKQSGRTASL

Nucleotide

• Length: 468 bp

>AT144893 NZ_CP062245:1407812-1407990 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Antitoxin

• AlphaFold DB: A0A0X8LPP8