Detailed information of TA system TA144890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 524093..524859
• Replicon: chromosome
• Accession: NZ_CP046746
• Organism: Vibrio cholerae strain 3566-08

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9K2P7
• Locus tag: GPW95_RS16825
• Protein ID: WP_000982260.1
• Coordinates: 524093..524590 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GPW95_RS16830
• Protein ID: WP_019829878.1
• Coordinates: 524587..524859 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW95_RS16795	519320..519709	+	390	WP_000491261.1	VOC family protein	-
GPW95_RS16800	519861..520292	+	432	WP_229767297.1	DUF1993 family protein	-
GPW95_RS16805	520485..521258	+	774	WP_139284613.1	hypothetical protein	-
GPW95_RS16810	521399..521830	+	432	WP_044128141.1	hypothetical protein	-
GPW95_RS16815	521957..522412	+	456	WP_001245329.1	GNAT family N-acetyltransferase	-
GPW95_RS19290	522918..522980	+	63	Protein_474	acetyltransferase	-
GPW95_RS16820	522999..523958	+	960	WP_001887517.1	restriction endonuclease	-
GPW95_RS16825	524093..524590	-	498	WP_000982260.1	GNAT family N-acetyltransferase	Toxin
GPW95_RS16830	524587..524859	-	273	WP_019829878.1	DUF1778 domain-containing protein	Antitoxin
GPW95_RS16835	525849..526019	+	171	WP_000483444.1	DUF645 family protein	-
GPW95_RS16840	526224..526721	+	498	WP_005526565.1	GNAT family N-acetyltransferase	-
GPW95_RS19295	526777..526839	+	63	Protein_480	acetyltransferase	-
GPW95_RS16845	526856..527494	+	639	WP_019829928.1	hypothetical protein	-
GPW95_RS16850	527648..528226	+	579	WP_032473189.1	DJ-1/PfpI family protein	-
GPW95_RS16855	528354..528644	-	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GPW95_RS16860	528634..528882	-	249	WP_019829909.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GPW95_RS16865	529093..529746	+	654	WP_019829910.1	peptidoglycan DD-metalloendopeptidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	487525..541374	53849
	-	inside	Integron	-	-	490782..540962	50180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-89)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18527.18 Da
• Isoelectric Point: 8.7753

>T144890 WP_000982260.1 NZ_CP046746:c524590-524093 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T144890 NZ_CP062245:c1264730-1264623 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9713.24 Da
• Isoelectric Point: 6.2988

>AT144890 WP_019829878.1 NZ_CP046746:c524859-524587 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQTDAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT144890 NZ_CP062245:1264778-1264844 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Structures

Toxin

• AlphaFold DB: A0A0Q0KGB1