Detailed information of TA system TA144889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 515531..516029
• Replicon: chromosome
• Accession: NZ_CP046746
• Organism: Vibrio cholerae strain 3566-08

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: GPW95_RS16755
• Protein ID: WP_000589156.1
• Coordinates: 515531..515797 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: GPW95_RS16760
• Protein ID: WP_000643598.1
• Coordinates: 515784..516029 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW95_RS16720	510557..511093	+	537	WP_019829934.1	GNAT family protein	-
GPW95_RS16725	511397..511567	+	171	WP_000483444.1	DUF645 family protein	-
GPW95_RS16730	511772..512242	+	471	WP_032473170.1	GNAT family N-acetyltransferase	-
GPW95_RS16735	512407..513147	+	741	WP_161779866.1	PhzF family phenazine biosynthesis protein	-
GPW95_RS16740	513286..513822	+	537	WP_071181460.1	AAA family ATPase	-
GPW95_RS16745	513960..514718	+	759	WP_001887513.1	hypothetical protein	-
GPW95_RS16750	514963..515223	+	261	WP_229767299.1	DUF3709 domain-containing protein	-
GPW95_RS19285	515412..515510	+	99	WP_229767298.1	acetyltransferase	-
GPW95_RS16755	515531..515797	+	267	WP_000589156.1	BrnT family toxin	Toxin
GPW95_RS16760	515784..516029	+	246	WP_000643598.1	hypothetical protein	Antitoxin
GPW95_RS16765	516173..516589	+	417	WP_019830302.1	lysozyme inhibitor LprI family protein	-
GPW95_RS16770	516745..517281	+	537	WP_019829641.1	nucleotidyltransferase family protein	-
GPW95_RS16775	517338..517532	+	195	WP_154081050.1	DUF3709 domain-containing protein	-
GPW95_RS16780	517550..517810	+	261	WP_032473168.1	DUF3709 domain-containing protein	-
GPW95_RS16785	518072..518512	+	441	WP_032473167.1	lysozyme inhibitor LprI family protein	-
GPW95_RS16790	518664..519116	+	453	WP_032473166.1	GNAT family N-acetyltransferase	-
GPW95_RS16795	519320..519709	+	390	WP_000491261.1	VOC family protein	-
GPW95_RS16800	519861..520292	+	432	WP_229767297.1	DUF1993 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	487525..541374	53849
	-	inside	Integron	-	-	490782..540962	50180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T144889 WP_000589156.1 NZ_CP046746:515531-515797 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T144889 NZ_CP062245:c475764-475546 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT144889 WP_000643598.1 NZ_CP046746:515784-516029 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT144889 NZ_CP062245:c476164-475790 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2