Detailed information of TA system TA144878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 659459..659957
• Replicon: chromosome
• Accession: NZ_CP046743
• Organism: Vibrio cholerae strain 3569-08

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: GPW72_RS03375
• Protein ID: WP_000589156.1
• Coordinates: 659691..659957 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: GPW72_RS03370
• Protein ID: WP_000643598.1
• Coordinates: 659459..659704 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW72_RS03340	655072..655431	-	360	WP_001071529.1	VOC family protein	-
GPW72_RS03345	655585..656175	-	591	WP_000021480.1	hypothetical protein	-
GPW72_RS03350	656315..656740	-	426	WP_000403005.1	GNAT family N-acetyltransferase	-
GPW72_RS03355	656937..657119	-	183	WP_000923182.1	DUF645 family protein	-
GPW72_RS03360	657407..658411	-	1005	WP_000964920.1	LD-carboxypeptidase	-
GPW72_RS03365	658569..659363	-	795	WP_001911581.1	hypothetical protein	-
GPW72_RS03370	659459..659704	-	246	WP_000643598.1	hypothetical protein	Antitoxin
GPW72_RS03375	659691..659957	-	267	WP_000589156.1	BrnT family toxin	Toxin
GPW72_RS03380	660142..660804	-	663	WP_000960654.1	hypothetical protein	-
GPW72_RS03385	660932..661399	-	468	WP_001289288.1	OsmC family protein	-
GPW72_RS19220	661611..661649	-	39	WP_082798268.1	hypothetical protein	-
GPW72_RS19225	661703..661846	-	144	Protein_646	DUF645 family protein	-
GPW72_RS03395	662221..662499	-	279	WP_000280324.1	DUF3709 domain-containing protein	-
GPW72_RS03405	662737..663270	-	534	WP_000118351.1	GNAT family N-acetyltransferase	-
GPW72_RS03410	663267..663587	-	321	WP_001909340.1	DUF1778 domain-containing protein	-
GPW72_RS03415	663907..664128	-	222	WP_012705854.1	DUF3709 domain-containing protein	-
GPW72_RS03420	664149..664309	-	161	Protein_651	DUF3709 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	575627..724145	148518
	-	inside	Integron	-	-	575627..717201	141574

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T144878 WP_000589156.1 NZ_CP046743:c659957-659691 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T144878 NZ_CP062244:c3161903-3161607 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT144878 WP_000643598.1 NZ_CP046743:c659704-659459 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT144878 NZ_CP062244:c3161605-3161210 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2