Detailed information of TA system TA144875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 608420..608948
• Replicon: chromosome
• Accession: NZ_CP046743
• Organism: Vibrio cholerae strain 3569-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: GPW72_RS02905
• Protein ID: WP_000221352.1
• Coordinates: 608658..608948 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H9L4T4
• Locus tag: GPW72_RS02900
• Protein ID: WP_000213183.1
• Coordinates: 608420..608668 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW72_RS02855	603459..603923	+	465	WP_000140244.1	hypothetical protein	-
GPW72_RS02860	604023..604421	-	399	WP_001882293.1	hypothetical protein	-
GPW72_RS02865	604577..605077	-	501	WP_000789723.1	hypothetical protein	-
GPW72_RS02870	605215..605745	-	531	WP_000415563.1	hypothetical protein	-
GPW72_RS02875	605896..606291	-	396	WP_000649527.1	DUF3465 domain-containing protein	-
GPW72_RS02880	606499..606924	-	426	WP_000415748.1	hypothetical protein	-
GPW72_RS02885	607196..607849	-	654	WP_000036957.1	site-specific DNA-methyltransferase	-
GPW72_RS02900	608420..608668	+	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GPW72_RS02905	608658..608948	+	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPW72_RS02910	609084..609599	-	516	WP_001201532.1	lipocalin family protein	-
GPW72_RS02915	609736..610212	-	477	WP_001047180.1	GNAT family N-acetyltransferase	-
GPW72_RS02920	610240..610350	-	111	WP_071917832.1	DUF3265 domain-containing protein	-
GPW72_RS02925	610347..611276	-	930	WP_000335802.1	hypothetical protein	-
GPW72_RS02930	611396..611842	-	447	WP_000128977.1	hypothetical protein	-
GPW72_RS02935	611995..612999	-	1005	WP_000964925.1	LD-carboxypeptidase	-
GPW72_RS19185	613212..613250	-	39	WP_106019119.1	hypothetical protein	-
GPW72_RS19190	613266..613391	-	126	WP_001944767.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	575627..724145	148518
	-	inside	Integron	-	-	575627..717201	141574

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T144875 WP_000221352.1 NZ_CP046743:608658-608948 [Vibrio cholerae]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T144875 NZ_CP062244:2770812-2771141 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8937.28 Da
• Isoelectric Point: 4.1079

>AT144875 WP_000213183.1 NZ_CP046743:608420-608668 [Vibrio cholerae]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT144875 NZ_CP062244:2770474-2770791 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A366A3W8