Detailed information of TA system TA144865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2255157..2256985
• Replicon: chromosome
• Accession: NZ_CP046742
• Organism: Vibrio cholerae strain 3523-03

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GPW86_RS16360
• Protein ID: WP_165387912.1
• Coordinates: 2255624..2256985 (-)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GPW86_RS16355
• Protein ID: WP_029786578.1
• Coordinates: 2255157..2255639 (-)
• Length: 161 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW86_RS16350	2251567..2254839	+	3273	WP_123011384.1	type I restriction endonuclease subunit R	-
GPW86_RS16355	2255157..2255639	-	483	WP_029786578.1	helix-turn-helix domain-containing protein	Antitoxin
GPW86_RS16360	2255624..2256985	-	1362	WP_165387912.1	type II toxin-antitoxin system HipA family toxin	Toxin
GPW86_RS16370	2257780..2258265	-	486	WP_000162689.1	SsrA-binding protein SmpB	-
GPW86_RS16375	2258449..2258883	+	435	WP_000815880.1	type II toxin-antitoxin system RatA family toxin	-
GPW86_RS16380	2258886..2259191	+	306	WP_001918351.1	RnfH family protein	-
GPW86_RS16385	2259269..2259628	-	360	WP_001160907.1	outer membrane protein assembly factor BamE	-
GPW86_RS16390	2259787..2261451	-	1665	WP_123011385.1	DNA repair protein RecN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-385)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-139)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51397.15 Da
• Isoelectric Point: 4.8182

>T144865 WP_165387912.1 NZ_CP046742:c2256985-2255624 [Vibrio cholerae]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHHDKDDFHAVSLNHPVSFFFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSNHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIEQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPVYDFAPMKADPEGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEVLLQELEVTASKCLDLKQRLALRGVPEQILEMPAVGLNHISEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T144865 NZ_CP062244:c1265801-1265694 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 161 a.a.
• Molecular weight: 17535.05 Da
• Isoelectric Point: 10.8446

>AT144865 WP_029786578.1 NZ_CP046742:c2255639-2255157 [Vibrio cholerae]
MGATMNKPSNKESVQDTVARLRANRNQHQPKTGTDATHSTKERSQLNTPTRSKTSKNAKAVSAAERKTEANKIIKHLLLG
ELTQGQALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFGLKVGMLPSSPDVLKSLLIDGEAG

Nucleotide

• Length: 483 bp

>AT144865 NZ_CP062244:1265848-1265914 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT