Detailed information of TA system TA144863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 627424..627989
• Replicon: chromosome
• Accession: NZ_CP046741
• Organism: Vibrio cholerae strain 3523-03

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D7HCW8
• Locus tag: GPW86_RS03540
• Protein ID: WP_000869999.1
• Coordinates: 627702..627989 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D0IIK3
• Locus tag: GPW86_RS03535
• Protein ID: WP_000086649.1
• Coordinates: 627424..627705 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW86_RS03500	622854..623036	+	183	WP_123011680.1	DUF645 family protein	-
GPW86_RS03505	623235..623741	+	507	WP_123011681.1	GNAT family N-acetyltransferase	-
GPW86_RS03510	623885..624391	+	507	WP_123011681.1	GNAT family N-acetyltransferase	-
GPW86_RS03515	624535..625071	+	537	WP_095474066.1	hypothetical protein	-
GPW86_RS03520	625220..625696	+	477	WP_102787218.1	hypothetical protein	-
GPW86_RS03525	625719..626678	-	960	WP_123011654.1	IS30 family transposase	-
GPW86_RS03535	627424..627705	+	282	WP_000086649.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GPW86_RS03540	627702..627989	+	288	WP_000869999.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPW86_RS03545	628136..628606	-	471	WP_000784954.1	RES family NAD+ phosphorylase	-
GPW86_RS03550	628606..629058	-	453	WP_000024295.1	DUF2384 domain-containing protein	-
GPW86_RS03560	629363..629650	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GPW86_RS03565	629661..629978	+	318	WP_001232701.1	HigA family addiction module antidote protein	-
GPW86_RS03570	630120..630500	+	381	WP_113601670.1	MmcQ/YjbR family DNA-binding protein	-
GPW86_RS03575	630536..631480	-	945	WP_038964128.1	IS110 family transposase	-
GPW86_RS03580	631680..631793	+	114	WP_123011836.1	DUF3265 domain-containing protein	-
GPW86_RS03585	631820..632293	+	474	WP_123011684.1	GrpB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	443489..637173	193684
	-	flank	IS/Tn	-	-	630536..631480	944
	-	inside	Integron	-	-	573974..638391	64417

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-74)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10997.74 Da
• Isoelectric Point: 6.0710

>T144863 WP_000869999.1 NZ_CP046741:627702-627989 [Vibrio cholerae]
MKVVWSPLALQKLGDAAEFIALDNPSAAEKWVNEVFDKTELLGSMPEMGRMVPEMPHTNYREIIFGHYRIIYSLSHEIRV
LTVRNCRQLLTEHDV

Nucleotide

• Length: 288 bp

>T144863 NZ_CP062244:c1264731-1264624 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10361.93 Da
• Isoelectric Point: 5.1548

>AT144863 WP_000086649.1 NZ_CP046741:627424-627705 [Vibrio cholerae]
MSRIHLDQDIQPLSEFRAGVASFIKQINETRRPLVITQRGKGVAVVLDVAEYEAMQEKIELLEEMRTAEAQLAAGLGISN
EDARSQVLGRIIK

Nucleotide

• Length: 282 bp

>AT144863 NZ_CP062244:1264779-1264845 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Structures

Toxin

• AlphaFold DB: A0A0H3AEF1

Antitoxin

• AlphaFold DB: A0A0K9UIW4