Detailed information of TA system TA144862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-Phd
• Location: 620641..621219
• Replicon: chromosome
• Accession: NZ_CP046741
• Organism: Vibrio cholerae strain 3523-03

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: GPW86_RS03470
• Protein ID: WP_184476341.1
• Coordinates: 620641..620958 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A366AI09
• Locus tag: GPW86_RS03475
• Protein ID: WP_000557291.1
• Coordinates: 620977..621219 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW86_RS19390	615798..615977	+	180	WP_174500466.1	DUF645 family protein	-
GPW86_RS03440	616023..616061	+	39	WP_108315918.1	hypothetical protein	-
GPW86_RS03445	616267..616731	+	465	WP_032473198.1	ASCH domain-containing protein	-
GPW86_RS03450	617134..618093	-	960	WP_123011654.1	IS30 family transposase	-
GPW86_RS03455	618328..618843	+	516	WP_001894921.1	lipocalin family protein	-
GPW86_RS03460	618875..619834	-	960	WP_123011654.1	IS30 family transposase	-
GPW86_RS03465	620051..620506	+	456	WP_071180673.1	GNAT family N-acetyltransferase	-
GPW86_RS03470	620641..620958	-	318	WP_184476341.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPW86_RS03475	620977..621219	-	243	WP_000557291.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GPW86_RS03480	621608..621704	+	97	Protein_657	DUF645 family protein	-
GPW86_RS03485	621723..621761	+	39	WP_108244118.1	hypothetical protein	-
GPW86_RS03490	621939..622256	-	318	WP_123011679.1	CcdB family protein	-
GPW86_RS03495	622256..622501	-	246	WP_001260801.1	type II toxin-antitoxin system CcdA family antitoxin	-
GPW86_RS03500	622854..623036	+	183	WP_123011680.1	DUF645 family protein	-
GPW86_RS03505	623235..623741	+	507	WP_123011681.1	GNAT family N-acetyltransferase	-
GPW86_RS03510	623885..624391	+	507	WP_123011681.1	GNAT family N-acetyltransferase	-
GPW86_RS03515	624535..625071	+	537	WP_095474066.1	hypothetical protein	-
GPW86_RS03520	625220..625696	+	477	WP_102787218.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	443489..637173	193684
	-	inside	Integron	-	-	573974..638391	64417

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-93)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12198.01 Da
• Isoelectric Point: 9.7496

>T144862 WP_184476341.1 NZ_CP046741:c620958-620641 [Vibrio cholerae]
MQNKQYKLSHLAQEHLLKIKHYTIENFAETQWQKYKSTLLSGFQTLADNPGLGKSCEDIYQNGFYFPVGKHMAYYTKEAN
FILIVAVLGQSQLPQKHLKQSRFVS

Nucleotide

• Length: 318 bp

>T144862 NZ_CP062244:c475764-475546 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8931.22 Da
• Isoelectric Point: 5.6630

>AT144862 WP_000557291.1 NZ_CP046741:c621219-620977 [Vibrio cholerae]
MHTLTANDAKRNFGELLLSAQREPVIISKNSKNTVVVMSIKDFEELEAMKLDYLKHCFESAQKDLDSGKTVDGAAFLNTL

Nucleotide

• Length: 243 bp

>AT144862 NZ_CP062244:c476164-475790 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A366AI09