Detailed information of TA system TA144860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 570323..570833
• Replicon: chromosome
• Accession: NZ_CP046741
• Organism: Vibrio cholerae strain 3523-03

Toxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GPW86_RS03065
• Protein ID: WP_184476392.1
• Coordinates: 570570..570833 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: GPW86_RS03060
• Protein ID: WP_123011657.1
• Coordinates: 570323..570577 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW86_RS03025	565811..566479	+	669	WP_123011656.1	hypothetical protein	-
GPW86_RS03030	566625..567011	+	387	WP_000703163.1	VOC family protein	-
GPW86_RS03035	567181..567552	+	372	WP_001008376.1	hypothetical protein	-
GPW86_RS03040	567711..568094	+	384	WP_029628683.1	hypothetical protein	-
GPW86_RS03045	568253..569053	+	801	WP_002045689.1	PhzF family phenazine biosynthesis protein	-
GPW86_RS03055	569171..569617	-	447	WP_076025899.1	BLUF domain-containing protein	-
GPW86_RS03060	570323..570577	+	255	WP_123011657.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
GPW86_RS03065	570570..570833	+	264	WP_184476392.1	Txe/YoeB family addiction module toxin	Toxin
GPW86_RS03070	571203..571817	-	615	WP_000092503.1	Fic family protein	-
GPW86_RS03075	571900..572844	-	945	WP_038964128.1	IS110 family transposase	-
GPW86_RS03080	573044..573157	+	114	WP_123011830.1	DUF3265 domain-containing protein	-
GPW86_RS03085	573974..574933	-	960	WP_123011654.1	IS30 family transposase	-
GPW86_RS03090	575087..575261	+	175	Protein_584	acetyltransferase	-
GPW86_RS19380	575378..575474	+	97	Protein_585	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	443489..637173	193684
	-	flank	IS/Tn	-	-	571900..572844	944
	-	inside	Integron	-	-	444653..571022	126369
	-	inside	Integron	-	-	573974..638391	64417

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10427.80 Da
• Isoelectric Point: 7.9816

>T144860 WP_184476392.1 NZ_CP046741:570570-570833 [Vibrio cholerae]
MISRLLSWTDESWDDYLYWQTQDKKTLKRINKIINDVKRSPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDNAITV
ISCRYHY

Nucleotide

• Length: 264 bp

>T144860 NZ_CP062244:248788-249186 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9423.62 Da
• Isoelectric Point: 5.0334

>AT144860 WP_123011657.1 NZ_CP046741:570323-570577 [Vibrio cholerae]
MRIVSFTEARNSLKAVLDAVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPANAEHLNRSIAQFKAGKVTQRD
LIDD

Nucleotide

• Length: 255 bp

>AT144860 NZ_CP062244:248492-248785 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA