Detailed information of TA system TA144859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 565046..565605
• Replicon: chromosome
• Accession: NZ_CP046741
• Organism: Vibrio cholerae strain 3523-03

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D7HHK2
• Locus tag: GPW86_RS03015
• Protein ID: WP_000578471.1
• Coordinates: 565046..565324 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GPW86_RS03020
• Protein ID: WP_076008603.1
• Coordinates: 565321..565605 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW86_RS02980	560544..561503	-	960	WP_123011654.1	IS30 family transposase	-
GPW86_RS19375	561658..561720	+	63	Protein_563	acetyltransferase	-
GPW86_RS02985	561742..562521	+	780	WP_123011587.1	alpha/beta hydrolase	-
GPW86_RS02990	562669..563625	+	957	WP_000057239.1	DUF4263 domain-containing protein	-
GPW86_RS02995	563586..563732	+	147	WP_123011829.1	DUF3265 domain-containing protein	-
GPW86_RS03000	563761..564165	+	405	WP_123011640.1	DUF1801 domain-containing protein	-
GPW86_RS03005	564295..564594	-	300	WP_033932139.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GPW86_RS03010	564591..564833	-	243	WP_001107719.1	type II toxin-antitoxin system ParD family antitoxin	-
GPW86_RS03015	565046..565324	-	279	WP_000578471.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
GPW86_RS03020	565321..565605	-	285	WP_076008603.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GPW86_RS03025	565811..566479	+	669	WP_123011656.1	hypothetical protein	-
GPW86_RS03030	566625..567011	+	387	WP_000703163.1	VOC family protein	-
GPW86_RS03035	567181..567552	+	372	WP_001008376.1	hypothetical protein	-
GPW86_RS03040	567711..568094	+	384	WP_029628683.1	hypothetical protein	-
GPW86_RS03045	568253..569053	+	801	WP_002045689.1	PhzF family phenazine biosynthesis protein	-
GPW86_RS03055	569171..569617	-	447	WP_076025899.1	BLUF domain-containing protein	-
GPW86_RS03060	570323..570577	+	255	WP_123011657.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	443489..637173	193684
	-	inside	Integron	-	-	444653..571022	126369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10838.56 Da
• Isoelectric Point: 4.6794

>T144859 WP_000578471.1 NZ_CP046741:c565324-565046 [Vibrio cholerae]
MIFWEEASLNDREKIFEFLYDFNPAAAEKTDELIEAKVENLLEQPLMGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Nucleotide

• Length: 279 bp

>T144859 NZ_CP062244:c242726-242448 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10929.26 Da
• Isoelectric Point: 8.0793

>AT144859 WP_076008603.1 NZ_CP046741:c565605-565321 [Vibrio cholerae]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKARIRNRGHA

Nucleotide

• Length: 285 bp

>AT144859 NZ_CP062244:c242989-242729 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A271VKS2