Detailed information of TA system TA144858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 564295..564833
• Replicon: chromosome
• Accession: NZ_CP046741
• Organism: Vibrio cholerae strain 3523-03

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: GPW86_RS03005
• Protein ID: WP_033932139.1
• Coordinates: 564295..564594 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P58093
• Locus tag: GPW86_RS03010
• Protein ID: WP_001107719.1
• Coordinates: 564591..564833 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW86_RS02970	559366..559596	-	231	WP_000838533.1	PAS factor family protein	-
GPW86_RS02975	559830..560507	+	678	WP_123011653.1	hypothetical protein	-
GPW86_RS02980	560544..561503	-	960	WP_123011654.1	IS30 family transposase	-
GPW86_RS19375	561658..561720	+	63	Protein_563	acetyltransferase	-
GPW86_RS02985	561742..562521	+	780	WP_123011587.1	alpha/beta hydrolase	-
GPW86_RS02990	562669..563625	+	957	WP_000057239.1	DUF4263 domain-containing protein	-
GPW86_RS02995	563586..563732	+	147	WP_123011829.1	DUF3265 domain-containing protein	-
GPW86_RS03000	563761..564165	+	405	WP_123011640.1	DUF1801 domain-containing protein	-
GPW86_RS03005	564295..564594	-	300	WP_033932139.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPW86_RS03010	564591..564833	-	243	WP_001107719.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
GPW86_RS03015	565046..565324	-	279	WP_000578471.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
GPW86_RS03020	565321..565605	-	285	WP_076008603.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
GPW86_RS03025	565811..566479	+	669	WP_123011656.1	hypothetical protein	-
GPW86_RS03030	566625..567011	+	387	WP_000703163.1	VOC family protein	-
GPW86_RS03035	567181..567552	+	372	WP_001008376.1	hypothetical protein	-
GPW86_RS03040	567711..568094	+	384	WP_029628683.1	hypothetical protein	-
GPW86_RS03045	568253..569053	+	801	WP_002045689.1	PhzF family phenazine biosynthesis protein	-
GPW86_RS03055	569171..569617	-	447	WP_076025899.1	BLUF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	443489..637173	193684
	-	inside	Integron	-	-	444653..571022	126369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11603.31 Da
• Isoelectric Point: 9.9232

>T144858 WP_033932139.1 NZ_CP046741:c564594-564295 [Vibrio cholerae]
MKPFNLTIAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKFPQGSHVIFYQQTGSQQ
IRVIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T144858 NZ_CP062243:c4570864-4570523 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8964.84 Da
• Isoelectric Point: 4.1677

>AT144858 WP_001107719.1 NZ_CP046741:c564833-564591 [Vibrio cholerae]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLIEGEQSGDADYDLDSFINELDSENIR

Nucleotide

• Length: 243 bp

>AT144858 NZ_CP062243:4570859-4570935 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin

• PDB: 7R5A
• PDB: 7B22
• AlphaFold DB: A0A151JFU4